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Molecular marker related to weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and acquisition method and application thereof

A molecular marker, Gaoyou duck technology, applied in the field of poultry genetic engineering, can solve the problems that have not yet been found, and achieve the effects of low cost, increased economic benefits, and increased body weight

Inactive Publication Date: 2016-02-10
JIANGSU INST OF POULTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, no molecular markers and applications related to Gaoyou duck body weight, breast leg muscle weight and pectoral muscle fiber traits have been found in the prior art.

Method used

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  • Molecular marker related to weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and acquisition method and application thereof
  • Molecular marker related to weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and acquisition method and application thereof
  • Molecular marker related to weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and acquisition method and application thereof

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Embodiment Construction

[0013] The present invention will be explained in detail below in conjunction with the embodiments.

[0014] The present invention is related to the molecular markers of Gaoyou duck body weight, pectoral leg muscle weight and pectoral muscle fiber traits, the molecular markers are located at the 190th position of the nucleotide shown in SEQ ID NO: 1, and the nucleotide at this site is G or T , resulting in a single nucleotide polymorphism at the gene locus. Molecular markers related to body weight, pectoralis muscle weight and pectoralis muscle fiber traits of Gaoyou duck were amplified by PCR. The nucleotide sequence was the partial genome sequence of duck IGF-1, and the sequence was shown in SEQ ID NO:1. There is a G190-T190 mutation at 190bp in the sequence, resulting in a single nucleotide polymorphism at this site.

[0015] For the single nucleotide polymorphism at the above site, the present invention also discloses its acquisition method, wherein the sequence of the fo...

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Abstract

The invention discloses a molecular marker related to weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and an acquisition method and application thereof. The molecular marker is positioned at the 190th locus of a nucleotide shown as SEQ ID NO:1, and the nucleotide at the locus is G or T. The genome DNA of the Gaoyou ducks is subjected to PCR amplification, the amplification product is sequenced after a ligase detection reaction, and the molecular marker related to the weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks is acquired by virtue of sequence analysis. The molecular marker is applied to screening the weight, breast and leg muscle weight and breast muscle fiber characteristics of Gaoyou ducks and can be used for early selection during family selection, and a molecular basis is provided during selection, so that the weight of the Gaoyou ducks while coming into the market is improved, and the economic benefits are increased.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering, and more specifically relates to a molecular marker related to Gaoyou duck body weight, breast leg muscle weight and breast muscle fiber traits, its acquisition method and application. Background technique [0002] Insulin growth factor-1 (IGF-1), a member of the insulin family, is one of the important genes in the growth axis of animals, and has the function of promoting cell proliferation and individual development. IGF-1 can promote protein synthesis, muscle stem cell proliferation, myoblast differentiation and myotube fusion through the IGFs receptors on target organs, thereby promoting the development of skeletal muscle. Experiments have found that in the embryonic stage of ducklings, injecting exogenous IGF-1 in ovo can significantly increase the body weight and breast muscle weight of ducklings after hatching, indicating that IGF-I can promote the growth of ducklings. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 朱春红陶志云宋卫涛徐文娟李慧芳刘宏祥章双杰姬改革王晓峰
Owner JIANGSU INST OF POULTRY SCI
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