A Molecular Identification Method for the CMS Line of Dian-1 Three-line Hybrid Japonica Rice

Abstract

The invention discloses a method for molecular identification of the Dian-1 type three-line hybrid japonica rice sterile line. According to the method, the sterile gene in the mitochondrial genome in the cytoplasm and the restorer gene sequence in the nucleus of the Dian-1 type japonica rice CMS line are respectively designed. Two pairs of primers (csSPF and csSPR1) and (RFSPF2 and RFSPR21) were used for PCR amplification. According to the amplification results of the two pairs of primers, the sterile line of Dian-1 japonica rice could be accurately determined, and the It can be distinguished from maintainer lines, restorer lines and hybrids to achieve efficient, rapid and accurate identification of sterile lines. Compared with the traditional technology, the present invention has unique design, simple method and high accuracy, which can fully meet the current development requirements, make the molecular positioning of fertility genes more accurate, and promote the development of the field of gene molecular identification.

Description

technical field [0001] The patent of the invention belongs to the field of breeding in the field of agricultural biotechnology, and in particular relates to a molecular identification method for a Dian-1 type three-line hybrid japonica rice sterile line. [0002] technical background [0003] Rice is one of the most important food crops in my country. The breeding of hybrid rice has made great contributions to the solution of food production and food and clothing problems in my country. The basis for the effective use of rice heterosis is the selection and application of male sterile lines. One of them is cytoplasmic male sterility controlled by the interaction of nuclear genes and cytoplasmic genes, and the other is nuclear male sterility controlled by the interaction of genetics and the environment, including light-sensitive and temperature-sensitive male sterility. At present, there are mainly cytoplasmic male sterile lines in japonica rice that utilize nuclear-cytoplasmic...

AI Technical Summary

Problems solved by technology

The selection accuracy rate decreases rapidly with the increase of the recombination rate. In molecular-assisted selection breeding, if the selection accuracy rate is required to reach more than 90%, the recombination rate between the marker and the target gene must be less than 5%. When the recombination rate reaches 10%, , the accuracy of selection has dropped to 80%

[0005] On the other hand, due to the relative lag in the research on sterile genes, as far as the current molecular identification development technology is concerned, there is still a lack of a method and means that can quickly, effectively and accurately detect the sterile line of Dian-1 japonica rice

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