Simple method for fast extracting human fecal bacterium DNA

A fecal and rapid technology, applied in the field of biology, can solve the problems of low efficiency, difficulty, different quantity and purity of alcohol precipitation, etc., and achieve the effects of low cracking cost, less pollution, and low extraction cost.
CN105385682AActive Publication Date: 2016-03-09HANGZHOU GUHE INFORMATION TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HANGZHOU GUHE INFORMATION TECH CO LTD
Publication Date
2016-03-09

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Abstract

The invention discloses a simple method for fast extracting human fecal bacterium DNA. The simple method sequentially includes the following steps that hot-cold rupturing treatment is carried out on a human fecal sample to rupture cells; the DNA is captured through AMPure XP magnetic bead liquid; magnetic force adsorption of magnetic beads and a magnetic separation frame is used for removing impurities and purifying the DNA. According to the extracting method, as a physical method (liquid nitrogen freeze thawing) and magnetic bead purification are organically combined, one operator can extract about 100 samples at the same time within one hour, which has great significance in comprehensively and fast researching people group intestinal floras and related disease occurrence mechanisms.
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Description

technical field

[0001] The invention belongs to the fields of biology and medicine; in particular, it relates to a simple method for rapidly extracting bacterial DNA from human feces. Background technique

[0002] The intestinal microbial flora is a huge and complex ecosystem, which accounts for about 80% of the total number of microecological bacteria in the body. Contains 100 trillion kinds of microorganisms, involving 1000 types of bacteria, the number of which is 10 times the number of human cells in the immune system. These bacteria help the body digest food, produce vitamins to prevent diseases caused by bacteria in food, and stimulate the body. As research progressed, scientists discovered that gut microbes also play a key role in many chronic diseases and conditions, such as inflammation and obesity. There should also be a close relationship between the intestinal microbial flora and the host’s genetic factors. Human genes will have an important impact on the intes...

Claims

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