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A kind of method and dha to prepare dha by using bean dregs

A technology of enzymatic hydrolysis of bean dregs and bean dregs, which is applied in the field of DHA, can solve the problems of low DHA content, low utilization rate of bean dregs, and high production costs, and achieve the effects of increasing DHA content, realizing resource recycling, and increasing production

Active Publication Date: 2019-02-15
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, the first aspect of the embodiment of the present invention provides a method for preparing DHA from bean dregs to solve the problems of low utilization rate of existing bean dregs, environmental pollution, high production cost and low DHA content of existing DHA products

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  • A kind of method and dha to prepare dha by using bean dregs
  • A kind of method and dha to prepare dha by using bean dregs
  • A kind of method and dha to prepare dha by using bean dregs

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Embodiment 1

[0069] A kind of method utilizing bean dregs to prepare DHA, comprises the steps:

[0070] (1) Okara pretreatment

[0071] First dehydrate and dry the bean dregs until the water content of the bean dregs is about 20%, then crush them, pass through a 60-mesh sieve, and then expand them;

[0072] (2) Enzymatic hydrolysis of bean dregs

[0073] Weigh 40g of puffed dried okara to make okara homogenate at a solid-to-liquid mass ratio of 1:20, add a compound enzyme preparation with a mass content of not more than 0.3% to the okara homogenate, adjust pH to 4.0, and enzymatically hydrolyze for 24 hours at a temperature of 55°C , to obtain an enzymolysis mixture; the enzymolysis mixture is centrifuged to obtain sediment and bean dregs enzymolysis supernatant, and all bean dregs enzymolysis supernatants are collected for the next step of the fermentation production of Schizochytrium; the compound enzyme preparation is a volume ratio 1:1 cellulase and pectinase with enzyme activity rea...

Embodiment 2

[0082] The difference between this embodiment and embodiment 1 is that the concentration of bean dregs is 20g / L.

Embodiment 3

[0084] The difference between this embodiment and embodiment 1 is that the concentration of bean dregs is 60g / L.

[0085] The dry powders of Schizochytrium obtained in Examples 1-3 were respectively subjected to the following experimental determinations: take an appropriate amount of dry powder of Schizochytrium and add concentrated HCl to break the wall in a water bath at 60-70°C, extract the oil with n-hexane, and put it in a water bath at 55°C. Rotary evaporation to constant weight, measure its total oil yield; take appropriate amount of oil and internal standard (eicosanoic acid), according to the conventional BF 3 -Ether catalyst for KOH-CH 3 OH methyl esterification, and the DHA production in the bacteria was determined by gas chromatography.

[0086] The experimental results are shown in Table 1, figure 2 with image 3 shown.

[0087] Table 1 Fatty acid composition of Schizochytrium cultured at different concentrations of okara in the late stage of fermentation

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Abstract

The embodiment of the invention provides a method for preparing DHA through bean dregs. The method includes the following steps that 1, the bean dregs are pretreated; 2, enzymolysis is conducted on the bean dregs through a complex enzyme preparation composed of cellulase and cellulose, an enzymolysis mixture is obtained, and bean dreg enzymolysis liquid supernatant is obtained after centrifugation; 3, glucose and bean dreg enzymolysis liquid supernatant is dissolved in artificial sea water, a fermentation culture medium with bean dregs as a main nitrogen source is obtained, and the concentration of the bean dreg enzymolysis liquid supernatant is 20-60 g / L; 4, a fermentation tank system for controlling the dissolved oxygen amount in a staged mode is adopted to inoculate fission chytrid fungus seeds into the fermentation culture medium, stirring is conducted, air is introduced, fermentation is conducted for 4-6 days, and fermentation liquid is obtained; 5, fission chytrid fungus dry powder is obtained after centrifugal drying is conducted on the fermentation liquid, and the DHA is obtained. By means of the method, the production cost of the DHA can be remarkably lowered, meanwhile the bean dregs are changed to things of value, and pollution to the environment is reduced. The invention further provides the DHA prepared through the method.

Description

technical field [0001] The invention relates to the field of microbial fermentation production, in particular to a method for preparing DHA from bean dregs and DHA. Background technique [0002] Okara is a by-product in the process of producing soybean milk or tofu, and the global production of okara is huge every year. At present, people's utilization rate of bean dregs is low. The traditional use is to use bean dregs as animal feed and directly feed animals, so that the utilization rate of many high nutritional components in bean dregs is low. Some even directly discard bean dregs as waste, which is harmful to the environment caused pollution. Therefore, it is necessary to seek a new process that can efficiently utilize bean dregs and completely turn waste into treasure. [0003] DHA, full name docosahexaenoic acid, is an important omega-3 series polyunsaturated fatty acid. At present, the production of DHA by microbial fermentation has broad application prospects becau...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C07C57/03
Inventor 梁岩郭静刘春花
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI