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b-hemolytic streptococcus screening reagent and preparation method thereof, screening method for b-hemolytic streptococcus
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A screening technology for hemolytic streptococci, which is applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of high cost, complicated detection process, and long time, and achieve high accuracy, uniform color, The effect of reducing the cost of consumables
Active Publication Date: 2019-02-19
长春呈实健康实业有限公司
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[0004] The present invention solves the technical problems of complex detection process, long time and high cost of the existing hemolytic streptococcus detection method; and provides B hemolytic streptococcus screening reagent and screening method
Method used
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specific Embodiment approach 1
[0017] Embodiment 1. In this embodiment, the B-hemolytic streptococcus screening reagent is made of the following raw materials: serum broth (streptococcus broth): 5.0g, peptone: 30.0g, starch: 20.0g, beef extract : 4.0g, dextrose: 2.0g, magnesium sulfate: 0.02g, growth factor: 1.1g, agar: 4.5g, serum: 10ml and phosphate buffer: 1000ml;
[0018] Its preparation method is carried out according to the following steps:
[0019] The screening method for B-hemolytic streptococci is carried out in the following steps:
[0020] Step 1. Collect samples: collect vaginal or rectal secretions with a sterile swab;
[0021] Step 2. The sample after collection is directly transferred into the culture medium test tube equipped with the B-hemolytic streptococcus screening reagent described in claim 1, sealed, and sent to an incubator as soon as possible for 2 hours at 37° C. to observe the color of the reagent, and the discoloration is positive Sample, no color change is a negative sample; ...
specific Embodiment approach 2
[0024] Specific embodiment two: the screening method of B-hemolytic streptococcus in the present embodiment is carried out according to the following steps:
[0025] Step 1. Collect samples: use two sterile swabs to collect vaginal secretions and rectal secretions respectively;
[0026] Step 2. The collected samples were directly transferred into the culture medium test tubes equipped with the B-hemolytic streptococcus screening reagent described in Embodiment 1, sealed, and sent to an incubator as soon as possible for 2 h at 37° C. to observe the color of the reagent. A color change is a positive sample, and no color change is a negative sample; that is, the screening for B-hemolytic streptococci has been completed.
[0027] The B-hemolytic streptococcus screening reagent in this embodiment is a colorless gel with uniform color, tasteless, odorless, and no impurities; it is sealed and stored in a cool and dry place; the validity period is 2 years.
[0028] The invention can ...
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Abstract
The invention discloses a B hemolytic streptococcus screening reagent, a preparation method thereof and a B hemolytic streptococcus screening method, and belongs to the field of B hemolytic streptococcus screening. The technical problems that an existing hemolytic streptococcus screening method is complex in detection process, long in time and high in cost are solved. The B hemolytic streptococcus screening reagent is prepared by mixing serum broth, peptone, starch, a beef extract, glucose, magnesium sulfate, growth factor agar, serum and a phosphate buffer. The screening method comprises the steps of 1, collecting a sample; 2, directly moving the collected sample into a culture medium test tube of the B hemolytic streptococcus screening reagent specified in the claim 1, performing sealing, feeding the sample to an incubator as soon as possible at the temperature of 37 DEG C for culturing for 2-24 h, and observing the color of the reagent, wherein the sample is a positive sample if the color is changed and is a negative sample if the color is not changed. The B hemolytic streptococcus screening reagent is used for screening the B hemolytic streptococcus.
Description
technical field [0001] The invention belongs to the field of B-hemolytic streptococcus screening, and specifically relates to a screening reagent for hemolytic streptococcus, a preparation method and a screening method thereof. Background technique [0002] Group B hemolytic streptococcus is a Gram-positive coccus that mainly parasitizes the pathogenic bacteria in the urogenital tract and lower digestive tract of people. In recent years, the number of reproductive infections caused by group B hemolytic streptococcus has been increasing, especially the perinatal infection has been showing an upward trend. Therefore, the detection of group B hemolytic streptococci is of great significance. [0003] According to the national standard B-hemolytic streptococcus inspection process (GB4789.11-2014), the inspection process is complicated, time-consuming (18h-24h), and sample cost is high (serum 25g). Contents of the invention [0004] The invention aims to solve the technical pr...
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