Column unloading valve and automatic chromatography device with column unloading valve and device usage

Abstract

The invention discloses a column unloading valve and an automatic chromatography device containing the column unloading valve and the usage of the device. The column unloading valve includes a bottom plate, a valve body is fixedly arranged under the bottom plate, a central hole is arranged on the bottom plate, and the surrounding of the central hole is A ring hole is provided, and the ring hole communicates with the oblique interface on the valve body. The oblique interface is provided with a valve for opening / closing the oblique interface channel. The lower end of the central hole is provided with a plug that can move up and down. The connection between the plug and the central hole To realize the opening and closing of the valve body, the plug is connected with a hollow tube running through the valve body. The bottom end of the hollow tube is an interface. The outer wall of the hollow tube forms a channel with the inner wall of the valve body. The channel is perpendicular to The interfaces are connected, and the up and down movement of the hollow tube in the channel drives the up and down movement of the plug. The device is mainly used for the separation and purification of proteins in the development of blood coagulation factor products, and realizes the separation and purification of plasma proteins. The device has technical characteristics such as simple operation and simple separation steps, and can meet the needs of process characteristics.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a column unloading valve, an automatic chromatography device containing the column unloading valve and the usage of the device. Background technique [0002] The raw material of blood products is plasma. 92%-93% of human plasma is water, and the remaining 7%-8% is protein. Blood products are various special functional proteins separated and purified from this part of protein. [0003] The traditional plasma protein separation and collection equipment is mainly composed of barrel column, mesh cloth, stirring rod and liquid collection joint. When this type of equipment is used, the filler and the buffer are stirred and mixed manually, and then injected into the raw plasma. After the protein components are collected, the filler is also removed manually. Finally, the cleaning of the equipment is still done manually. Homogenization and barrel column cleaning are all by manpowe...

AI Technical Summary

Problems solved by technology

Homogenization and barrel column cleaning are all manual, and the protein is difficult to fully adsorb and lose more during the homogenization process

The cleaning of the barrel column is to manually dump the pure water in the barrel. This link is too laborious, the cleaning is not sufficient, and it is time-consuming. Moreover, the way of manpower driving the stirring rod limits the volume of the barrel column, which cannot adapt to industrialized mass production.

There is no top cover on the equipment, which belongs to open operation, and other substances are easy to intervene during operation, and the safety is low

[0004] At present, the domestic equipment that mainly relies on manual stirring to separate and purify proteins has poor uniformity of fillers, which directly affects the collection efficiency of protein components and is unstable.

There is no top cover on the device, and it belongs to open operation. It is easy for other media to enter during operation, and more cells are lost during separation, which is low in safety.

Therefore, it is not suitable for industrial mass production

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