Discharging column valve, automatic chromatography apparatus containing discharging column valve and apparatus usage method

Abstract

The invention discloses a discharging column valve, an automatic chromatography apparatus containing the discharging column valve and an apparatus usage method. The discharging column valve comprises a bottom plate, a valve body is fixedly disposed under the bottom plate, the bottom plate is provided with a center hole, annular holes are arranged at the periphery of the center hole and are communicated with an oblique joint on the valve body, and a valve for opening / closing the oblique-joint channel is arranged at the oblique joint; and the lower end of the center hole is provided with a vertically-movable plug, and matching of the plug and the center hole realizes opening and closing of the valve body; and the plug is connected with a hollow pipe penetrating the valve body, the lower end of the hollow pipe is a joint, a channel is formed by the outer wall of the hollow pipe and the inner wall of the valve body, and the channel is communicated with a vertical joint, and the hollow pipe vertically moves in the channel and drives the plug to move vertically. The apparatus is mainly applied to protein separation purification during blood-coagulation-factor product development, and realizes separation purification of plasma protein. The apparatus possesses the technical characteristics of simple operation, simple separation step and the like, and satisfies demands on technical characteristics.

Description

Technical field [0001] The invention relates to the technical field of biological engineering, in particular to an unloading column valve and an automatic chromatography device containing the unloading column valve and its usage. Background technique [0002] The raw material of blood products is plasma, 92%-93% of human plasma is water, and the remaining 7%-8% is protein. Blood products are a variety of special functional proteins separated and purified from this part of the protein. [0003] Traditional plasma protein separation and collection equipment is mainly composed of barrel column, mesh cloth, stirring rod and liquid collection joint. When this type of equipment is used, the filler and the buffer are stirred and mixed by manpower, and then injected into the raw plasma, the protein components are collected, the filler is also removed by manpower, and the final cleaning of the equipment is still manually cleaned. Homogenization and column cleaning rely on manpower. During ...

AI Technical Summary

Problems solved by technology

Homogenization and barrel column cleaning are all manual, and the protein is difficult to fully adsorb and lose more during the homogenization process

The cleaning of the barrel column is to manually dump the pure water in the barrel. This link is too laborious, the cleaning is not sufficient, and it is time-consuming. Moreover, the way of manpower driving the stirring rod limits the volume of the barrel column, which cannot adapt to industrialized mass production.

There is no top cover on the equipment, which belongs to open operation, and other substances are easy to intervene during operation, and the safety is low

[0004] At present, the domestic equipment that mainly relies on manual stirring to separate and purify proteins has poor uniformity of fillers, which directly affects the collection efficiency of protein components and is unstable.

There is no top cover on the device, and it belongs to open operation. It is easy for other media to enter during operation, and more cells are lost during separation, which is low in safety.

Therefore, it is not suitable for industrial mass production

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