A kind of bacillus amyloliquefaciens ws-8 and its application

A technology of amylolytic spores and WS-8, which is applied in the field of agricultural microorganisms and biology to achieve the effects of improving leaf color, promoting synthesis, and improving tomato quality

Active Publication Date: 2019-05-24
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the patents that have been authorized and published are Bacillus amyloliquefaciens for the control of soil-borne fungal diseases and post-harvest diseases of fruits. There are few related researches on the control of vegetable foliar fungal diseases, and there is no use of Bacillus amyloliquefaciens for the control of tomato leaf mold Related reports

Method used

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  • A kind of bacillus amyloliquefaciens ws-8 and its application
  • A kind of bacillus amyloliquefaciens ws-8 and its application

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Isolation and identification of Bacillus amyloliquefaciens WS-8

[0018] The broad-spectrum antibacterial Bacillus amyloliquefaciens WS-8 of the present invention can be obtained by collecting diseased and healthy vegetable leaves in tomato and cucumber planting sheds in Dahe Town, Luquan, Shijiazhuang, and cultivating them in the laboratory according to the following conditions:

[0019] Isolation and purification of strain WS-8: Healthy samples and diseased leaves after removal of rot and mold were used, and 10 grams of leaves were put into phosphate buffer solution by gradient dilution method, shaken at room temperature 200r / min for 30min, and ultrasonically treated for 5min. The bacterial suspension was coated on a plate, and a single colony was picked and purified by streaking.

[0020] Screening of antagonistic strain WS-8: the purified single colony was used for primary screening and re-screening of resistant strains with tomato leaf mold and early ...

Embodiment 2

[0027] Embodiment 2: the antibacterial spectrum determination of bacillus amyloliquefaciens WS-8

[0028] The bacterial strain of the present invention, with air-borne fungal disease: pear ring spot, pear black spot, cucumber gray mold, tomato leaf mold, tomato gray mold, tomato early blight, tomato soot; soil-borne fungal disease : blight, fusarium wilt, verticillium wilt, splinter blight; food-borne bacteria: staphylococcus aureus, a variety of pathogenic strains were carried out to measure the antibacterial spectrum, the results are shown in Table 2.

[0029] Table 2 Antibacterial spectrum of Bacillus amyloliquefaciens subspecies WS-8

[0030]

[0031] Note: The strength of antagonism is represented by "+''. "+": weak, the width of the inhibition zone is less than 1.5cm; "++'': medium strength, the width of the inhibition zone is between 1.5cm and 3.0cm;" +++": Very strong, with a width of the antibacterial zone greater than 3.0cm.

Embodiment 3

[0032] Embodiment 3: the cultivation and fermentation condition of bacillus amyloliquefaciens WS-8

[0033] Purify the strain with strong antibacterial effect, use beef extract peptone medium, and store it on a solid slant; pick a ring from the slant culture, inoculate it into the liquid medium of beef extract peptone and cultivate overnight at 37°C, as the strain seed bottle ready to use.

[0034]Fermentation culture conditions: fermentation medium: starch 0.7%, bean cake powder 1.6%, sucrose 0.2%, yeast extract 0.08%, peptone 0.05%, ammonium sulfate 0.15%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.03%, manganese sulfate 0.01 %, sodium chloride 0.02%, calcium carbonate 0.1%, pH7.3-7.5, the above are mass percentages. Sterilize at 121°C for 20 minutes, after cooling, add the seed liquid to the fermentation medium with an inoculation amount of 1.5-2%, in a 600mL / 3L Erlenmeyer flask, with a rotation speed of 180 rpm and a temperature of 30°C, and cultivate for 4...

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Abstract

The invention relates to a strain of Bacillus amyloliquefaciens subspecies WS-8 and its application, and discloses a subspecies of Bacillus amyloliquefaciens, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the number is CGMCC No.11787. The date is December 4, 2015. The invention also discloses the application of Bacillus amylobacter subspecies in the prevention and treatment of air-borne fungal diseases and soil-borne fungal diseases on the foliage of crops. The fermented liquid is diluted 50 times and sprayed once every 7-15 days, and the control effect on tomato leaf mold It can reach 72%, and it also has a good control effect on other air-borne and soil-borne fungal diseases; at the same time, the strain can also improve crop quality, effectively improve the leaf color of the plant, increase the content of chlorophyll, improve the disease resistance of the plant, and reduce pesticide residues , improve quality. The strain is convenient for artificial cultivation, has a fast propagation speed, is convenient for large-scale production, and has the potential of being developed into a biopesticide for preventing and controlling vegetable planting and post-harvest application.

Description

technical field [0001] The invention relates to a strain of Bacillus amyloliquefaciens WS-8 and its application, belonging to the field of agricultural microorganisms and biotechnology, and has the functions of improving leaf color, increasing leaf chlorophyll content, improving plant system disease resistance, preventing and controlling airborne fungal diseases on crop leaves, Efficacy to improve quality. Background technique [0002] The causative agent of leaf mold is Fulvia fulva (Cooke) Cif. , which belongs to the genus Bleusporium, synonymously known as Xanthosporium Cladosporium fulvum Cooke , is a common disease in protected tomato cultivation. The disease spreads quickly and causes serious damage. It generally causes a 20-30% reduction in yield, and can reach more than 50% in severe cases. At present, chemical pesticides are mainly used for prevention and control, but due to its complicated and complicated pathogenic differentiation, there are many physiological ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/07
CPCA01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 尹淑丽张丽萍刘洪伟梁然杨继业刘倩倩吕钊张根伟程辉彩
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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