A method for preserving the sperm of the black-tailed red catfish

A technology for the preservation method of black-tailed near-red tuna, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of short preservation time and high cost of sperm preservation effect, and achieves long preservation time, low cost, high cost and high cost. The effect of maintaining fertilization vitality

Active Publication Date: 2018-01-09
武汉先锋水产科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sperm preservation solution has the problems of short storage time and high cost for sperm preservation.

Method used

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Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0023] In order to improve the viability of sperm preservation in vitro and realize the protective development, the components of the sperm preservation solution are prepared with distilled water.

[0024] (1) Preparation of sperm preservation solution: weigh each raw material according to the ratio, dissolve them in distilled water, mix well, add distilled water to make up to 100 mL;

[0025] The sperm preservation solution is prepared by one of the following formulas:

[0...

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Abstract

The invention discloses an ancherythroculter nigrocauda sperm preservation method. The ancherythroculter nigrocauda sperm preservation method comprises the steps that 1, a sperm preserving fluid is prepared: raw material components are weighed in proportion and are respectively dissolved by using distilled water and are mixed evenly, and the distilled water is added to achieve volume fixing; 2, sperm acquisition is performed: an adult ancherythroculter nigrocauda milter is toweled off, and urine and faeces are removed to avoid sperm pollution; the abdomen is pressed slightly, extruded-out sperm is collected by using a suction tube, and sunlight or ultraviolet irradiation is avoided in the acquisition process; 3, sperm storage is performed: acquired ancherythroculter nigrocauda sperm is arranged in a dry, clean and sealed container subjected to sterilization and disinfection, and the container containing the sperm and the preserving fluid is kept in a low-temperature light-avoiding mode after the acquisition is completed. By the adoption of the technical scheme, the in-vitro survival time of the ancherythroculter nigrocauda sperm can be effectively prolonged, the fertilization vitality of the sperm can be kept, and protective development and distant crossbreeding of ancherythroculter nigrocauda are achieved. The ancherythroculter nigrocauda sperm preservation method is low in cost, simple and short in process and easy to operate, enables the preserving time to be long and has important application value.

Description

technical field [0001] The invention relates to the technical field of sperm preservation, in particular to a method for preserving the sperm of the red-tailed catfish. Background technique [0002] The black-tailed red carp is a unique economic fish in the upper reaches of the Yangtze River in my country. Due to overfishing, construction of water conservancy projects, etc., natural resources have seriously declined; artificial breeding is an important measure for resource protection and full utilization. In the process of artificial reproduction, the scales of P. nigra are easy to fall off and get injured, it is difficult to handle, and the survival rate of the parents is low; the sperm of P. nigra are stored for a short time after being excreted from the body. For example, at 4°C, 80% of them can be kept within 3 hours. The survival rate is only 50% within 7 hours. During the hybrid breeding process of the black-tailed red catfish, due to the difference in the breeding tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 王贵英李清祝东梅李佩陈见刘英武蔡汉玲
Owner 武汉先锋水产科技有限公司
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