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Suisi crabapple extract and active compound, preparation method and application

A kind of technology of succulent crabapple and extract, which is applied in the field of medicine and/or health care products to achieve good anticoagulant effect and good anticoagulant effect

Active Publication Date: 2019-08-06
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no extracts and compounds of Crabapple flower, kaempferol-3-O-α-L-arabinofuranoside, phloretin-4'-O-glucoside, kaempferol-3-O -Report on four effects of α-L-rhamnoside on blood coagulation in vitro in rabbits

Method used

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  • Suisi crabapple extract and active compound, preparation method and application
  • Suisi crabapple extract and active compound, preparation method and application
  • Suisi crabapple extract and active compound, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Extraction and Separation of Suisi Begonia Flower Extract and Active Compounds

[0022] A preparation method of the extract of the crabapple, which uses the flower of the crabapple as a raw material, immerses and extracts the flower of the crabapple dried in the shade with 70V% ethanol for 3 times, each time for 3 days, the extracts are combined, filtered, and concentrated to obtain the flower of the crabapple. Silk crabapple flower total extract. Take a part of the total extract of Begonia chinensis, dissolve it in methanol, separate it by D-101 macroporous resin column chromatography, and elute it with water, 20V% methanol, 40V% methanol, 60V% methanol, methanol gradient, and each concentration gradient elution 5 column volumes, each with a volume of 2000 mL, were concentrated to obtain water extract, 20% methanol extract, 40% methanol extract, 60% methanol extract, and methanol extract.

[0023] A method for isolating the active compound of Begonia chinens...

Embodiment 2

[0029] Example 2 The anticoagulant test of the extract and active compound of Crabapple flower

[0030] Method: Four tests of blood coagulation in vitro in rabbits

[0031] Instruments and materials: TGL-16gR high-speed desktop refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory); LRH-150 biochemical incubator (Shanghai Yiheng Technology Co., Ltd.); sodium chloride injection (Hebei Tiancheng Pharmaceutical Co., Ltd., A14091701); 0.109 mol / L sodium citrate solution (self-made), breviscapine for injection (Hunan Hengsheng Pharmaceutical Co., Ltd., 20110202); prothrombin time (PT) assay kit (105241), activated partial thrombin time (APTT) assay kit (112175), thrombin time (TT) assay kit (121132), fibrinogen (FIB) content assay kit (1320701) were all produced by Shanghai Sun Biotechnology Co., Ltd.

[0032] Experimental animals: Rex rabbit, male, weighing 2.0-2.5 kg, provided by the Institute of Traditional Chinese Medicine, School of Pharmacy, Henan University...

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Abstract

The invention belongs to the technical field of plant extracting, particularly relates to a filamental flowering crab extract comprising filamental flowering crab total extract, 40% of methyl alcohol part extract, 60% of methyl alcohol part extract and methyl alcohol part extract, as well as preparing and application of an active compound baohuoside II (1), kaempferol-3-O-alpha-L-furanoarabinoside (2), phloretin-4'-O-heteroside (3) and kaempferol-3-O-alpha-L-rhamnoside (4) separated from the filamental flowering crab. An experiment proves that each extract and each active compound of the filamental flowering crab have an anticoagulation action, and can be used for preparing anticoagulant singly or in a combining manner.

Description

technical field [0001] The invention belongs to the technical field of medicine and / or health care products, and specifically relates to an extract of crabapple, an active compound isolated from the flower of crabapple, a preparation method, and its new application in anticoagulation. Background technique [0002] Malus halliana Koehne (Malus halliana Koehne) is a plant of the genus Malus in the family Rosaceae. Chuisi crabapple has slight gas, slightly bitter and astringent taste, flat nature, belongs to the liver meridian, has the effect of regulating menstruation and blood, and is mainly used to treat metrorrhagia. Suisi Begonia, together with C. speciosa, Xifu Begonia (M. micromalus) and Papaya Begonia (C. cathayensis) of the same family, is called "Four Begonias". After reviewing the literature, it was found that: the crabapple has the functions of protecting the liver, anti-oxidation, and inhibiting the activity of α-glucosidase in vitro, and there are certain active ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/73C07H17/07C07H15/203C07H1/08A61K31/7048A61K31/7034A61P7/02
CPCA61K31/7034A61K31/7048A61K36/73A61K2236/333A61K2236/55C07H1/08C07H15/203C07H17/07A61K2300/00
Inventor 康文艺魏金凤王金梅崔丽丽余琦
Owner HENAN UNIVERSITY
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