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A gel for blood transfusion cross-matching, preparation method and experimental combiner

A cross-matching and combiner technology, applied in the field of blood-matching, can solve the problems of complicated cross-matching experiment process, etc., and achieve the effects of convenient and quick operation of blood transfusion cross-matching, accurate and reliable results, and high efficiency.

Inactive Publication Date: 2017-09-22
LUOHE MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a gel for blood transfusion and cross-matching, aiming at solving the complicated problem of the existing cross-matching experiment process

Method used

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  • A gel for blood transfusion cross-matching, preparation method and experimental combiner
  • A gel for blood transfusion cross-matching, preparation method and experimental combiner
  • A gel for blood transfusion cross-matching, preparation method and experimental combiner

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Embodiment Construction

[0039] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0040] The invention provides a disposable blood transfusion cross-matching experimental combiner, such as figure 1 As shown, the combiner is a shaped container, and the shaped container is a small transparent plastic test tube 1. The bottom of the test tube 1 is connected to the balloon 2 and the liquid outlet 3 through pipelines, and the top of the test tube 1 is connected to two tubes through pipelines. Liquid inlet 4,5.

[0041] Mix 100g sodium hyaluronate gel, 0.49g methyl p-hydrocarbyl benzoate, 0.26g sodium chloride, 2.0g sodium nitrite, 5.5g glucose, 0.70g edetate-2 sodium, 50U anti-human ...

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Abstract

The invention provides a gel for cross matching in blood transfusion, a preparation method, and an experiment combiner. The preparation method comprises the following steps: mixing 100 to 200 grams of sodium hyaluronate gel, 0.31 to 0.49 gram of p-alkyl methyl benzoate, 0.26 to 0.43 gram of sodium chloride, 1.5 to 2.0 grams of sodium nitrite, 5.5 to 7.0 grams of glucose, 0.65 to 0.70 gram of disodium ethylene diamine tetraacetic acid, 100 to 200 U of anti-human-blood immunoglobulin antibody, 0.8 to 0.95 gram of potassium dihydrogen phosphate, and 0.15 to 0.20 gram of disodium hydrogen phosphate; adding distilled water to a metered volume, adjusting the pH value to 6.6-6.8; stirring the mixed solution with a constant volume in vacuum at a temperature of 35 to 45 DEG C, taking out the solution, placing the solution in an experiment combiner, and carrying out moulding and refrigeration to obtain the gel. The provided gel has the advantages that the operation for cross matching becomes more convenient and quick, the result is accurate and reliable, the cross matching between bloods of two donors or more and blood of one acceptor can be carried out at the same time, and thus the efficiency is higher.

Description

technical field [0001] The invention belongs to the technical field of blood matching, and in particular relates to a gel for blood transfusion cross matching, a preparation method and an experiment combiner. Background technique [0002] The polybrene test uses a low-ion solution to promote the reaction of red blood cell antigens with antibodies in serum (plasma), and then uses polybrene to cause non-immune agglutination between red blood cells, shortening the distance between red blood cells that have reacted with antibodies (IgG). After the distance is shortened, it can cause the reacted antibody to react with other red blood cells to form immune agglutination. The non-immune erythrocyte agglutination reaction caused by polybrene can be neutralized and dispersed by the suspension added later. If there is an immune reaction, the agglutination will not be dispersed, which is a positive reaction. If there is no immune reaction, the agglutination will dissipate, which is a n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 何智宁尼亚李记超
Owner LUOHE MEDICAL COLLEGE
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