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A method for producing Cordyceps sinensis mycelium raw material by using mutagenic strains

A technology of Cordyceps sinensis mycelium and mutagenic strains is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., to achieve the effect of protecting public health and reducing production costs

Active Publication Date: 2019-04-02
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Inventor Liu Weimin has gradually realized that rice bran and bran as a fermentation medium need to be modified in many years of research to prevent the transfer of harmful substances such as lead, arsenic, and mercury in the original rice bran and bran to mycelia. , this issue has not been studied before the present invention

Method used

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  • A method for producing Cordyceps sinensis mycelium raw material by using mutagenic strains
  • A method for producing Cordyceps sinensis mycelium raw material by using mutagenic strains

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 4.8g / 100mL medium, and KH 2 PO 4 0.27g / 100mL, MgSO 4 ‘ 7H 2 O 0.27 g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use as medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 20 o C, rotating speed 180r / min, culture time 5d. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 5.6 g / 100mL medium, exopolysaccharide 449.3mg / 100mL medium, mycelia polysaccharide 277.3mg / 100mL medium, cordycepin content 2.6mg / g, lead content 1.45 mg / kg, the content of arsenic is 0.56mg / kg, and the content of mercury is 0.05mg / kg, all of ...

Embodiment 2

[0069] Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.0g / 100mL medium, the amount of bran used is 3.0g / 100mL medium, and KH 2 PO 4 0.015g / 100mL, MgSO 4 ‘ 7H 2 O 0.015 g / 100mL, cysteine ​​hydrochloride 20mg / 100mL, sodium copper chlorophyllin 5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a culture medium for fermentation, packed in shake flasks The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 23 o C, rotating speed 180r / min, culture time 5d. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results were: mycelium dry weight 3.4g / 100mL medium, exopolysaccharide 163.7mg / 100mL medium, mycelia polysaccharide 99.4mg / 100mL medium, cordycepin content 1.54mg / g, lead content 0.98 mg / kg, the content of arsenic is 0.38mg / kg, and the content of mercu...

Embodiment 3

[0071]Cordyceps sinensis CCTCC M 2015064 was used as the strain of Cordyceps sinensis. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 3.6g / 100mL medium, and KH 2 PO 4 0.14g / 100mL, MgSO 4 ‘ 7H 2 O 0.14 g / 100mL, cysteine ​​hydrochloride 35mg / 100mL, sodium copper chlorophyllin 7.5mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a medium for fermentation, shake The sample volume in the bottle is 40%, the inoculum volume is 10%, and the culture temperature is 20 o C, rotating speed 180r / min, culture time 6d. The following steps (3) to (9) in the technical solution described in the foregoing summary of the invention are implemented. The results are: mycelium dry weight 4.4 g / 100mL medium, exopolysaccharide 196.5mg / 100mL medium, mycelia polysaccharide 89.9mg / 100mL medium, cordycepin content 1.81mg / g, lead content 0.99mg / kg, the content of arsenic is 0.40mg / kg, and the content of mercury is 0.03mg / kg...

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Abstract

The invention discloses a method for producing a cordyceps sinensis mycelium raw material from a mutant strain and relates to the technical field of food microorganism applications. A new cordyceps sinensis strain obtained through ultraviolet mutagenesis is used for fermenting a new liquid-state rice bran complete feed culture medium with cysteine hydrochloride and sodium copper chlorophyll added, rice bran is efficiently converted into the cordyceps sinensis mycelium raw material, the production cost is reduced, the yield is increased, and the cordyceps sinensis mycelium raw material which has better quality and can be used for manufacturing health-care food is prepared. The low-value rice bran is converted into the cordyceps sinensis mycelium raw material with multiple health care functions, and the method has beneficial social and economic effects in the aspect of reduction of the production cost, efficient and reasonable utilization of low-end resources and protection of public health and has practicability.

Description

technical field [0001] The invention relates to the technical field of food microorganism application, in particular to a method for producing Cordyceps sinensis mycelium raw material by using a new strain of Cordyceps sinensis obtained by mutagenesis. In the method, the new strain is added with cysteine ​​hydrochloride and sodium copper chlorophyllin. Cordyceps sinensis mycelium with better quality is obtained by fermenting in the liquid rice bran bran whole material new medium with salt. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelium, fruiting bodies or spores of commonly used medicinal and edible fungi such as Cordyceps sinensis, Hericium erinaceus, Ganoderma lucidum, Grifola frondosa, etc. can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and antibiotics, or enriched with various trace el...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/04C12P19/40C12R1/645
CPCC12N1/14C12P19/04C12P19/40
Inventor 伍娟刘伟民王冲之王雪梅李婷婷程宇
Owner JIANGSU UNIV