Method for preparing tissue-engineered body from human autogeneic bone marrow-derived mesenchymal stem cells

Abstract

The invention discloses a method for preparing a tissue-engineered body from human autogeneic bone marrow-derived mesenchymal stem cells, belonging to the field of regenerative medicine. According to the invention, the autologous bone marrow of a patient is acquired under aseptic conditions and then subjected to stem cell separation and extraction and to culture and amplification so as to obtain a great number of the autogeneic bone marrow-derived mesenchymal stem cells; and the cells are subjected to structural shrinkage by using G-2 bone marrow mesenchymal stem cell special culturing technology so as to obtain the tissue-engineered body with a stable structure. The method is simple and practicable and has high repeatability.

Description

technical field [0001] The invention belongs to the technical field of regenerative medicine, and relates to the collection, separation, cultivation, expansion and freezing of bone marrow mesenchymal stem cells, and the construction of tissue engineering bodies by using the expanded bone marrow mesenchymal stem cells. Background technique [0002] The human body is composed of various tissues and organs, and different tissues play different roles in the human body. As people age, the functions of various tissues will gradually decline, making them unable to perform their normal functions. There are also some tissues and organs that are damaged due to certain diseases or external trauma, thus losing their functionality. For these reasons, tissues cannot provide normal physiological functions for the human body, which in turn affects other body functions. Therefore, repairing or replacing damaged tissue through external factors has become a way to treat tissue lesions or dec...

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Problems solved by technology

There are also clinically common skin transplantation operations. At present, artificial skin prepared from animal collagen tissue is commonly used. On the one hand, some risks may arise due to the source of animal tissue. The biological form is very different from its own skin tissue, and some infections will occur along with it

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