Application of rice gene BSK331 in improvement of plant stress tolerance

A technology for plant stress resistance and stress tolerance is applied in the application field of improving plant salt tolerance and drought, which can solve the problem of lack of detailed research on gene function, and achieve the effect of improving tolerance and having broad application prospects.

Inactive Publication Date: 2016-06-08
FRONTIER LAB OF SYST CROP DESIGN CO LTD +1
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Problems solved by technology

[0005] Rice has 11 genes encoding HAP3, the functions of which have not been studied in detail

Method used

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  • Application of rice gene BSK331 in improvement of plant stress tolerance
  • Application of rice gene BSK331 in improvement of plant stress tolerance
  • Application of rice gene BSK331 in improvement of plant stress tolerance

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Embodiment Construction

[0029] The methods used in the following examples are conventional methods unless otherwise specified, and the specific steps can be found in: "Molecular Cloning: A Laboratory Manual" (Sambrook, J., Russell, David W., Molecular Cloning: A Laboratory Manual, 3rdedition, 2001, NY, Cold Spring Harbor). The primers and DNA sequences used were synthesized by Shanghai Yingjun Biotechnology Co., Ltd.

[0030] 1. Separation of two genes of BSK331 and construction of expression vector

[0031] Primers were designed according to the sequence of the gene in GenBank, and the OsHAP3A and OsHAP3C genes were amplified by PCR using the 'plasmid containing the corresponding target gene preserved in the unit as a template. Its nucleotide sequence is shown in SEQ ID NO: 3 and 4 in the sequence listing, and the amino acid sequence of the encoded protein is shown in SEQ ID NO: 1 and 2 in the sequence listing.

[0032] 1) Primers for OsHAP3A gene:

[0033] Forward primer: 5'gaattcATGATGATGATGGATC...

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Abstract

The present invention discloses application of rice gene BSK331 in improvement of plant stress tolerance. Experiments show that the gene can significantly improve adversity stress high salt and drought tolerance when in use in conversion of rice. A protein and a coding gene have important theoretical and practical significance for study of plant stress tolerance mechanisms and improvement of plant stress tolerance and related traits, may play an important role in improvement of stress tolerance gene engineering of plants (especially cereals), and has broad application prospects.

Description

technical field [0001] The invention relates to genes related to plant stress tolerance and applications thereof, in particular to the application of transcription factors derived from rice and related to stress tolerance in improving plant salt and drought tolerance. Background technique [0002] Transcription factors (transcription factors, TF), also known as trans-acting factors, refer to DNA-binding protein molecules that can specifically interact with cis-acting elements in the promoter region of eukaryotic genes or can interact with these proteins. Related protein molecules, the main function is to specifically activate or inhibit the transcriptional effect of genes (Guilfoyle et al., 1997). They play an important regulatory role in the growth, development, morphogenesis, and environmental responses of biotic and abiotic stresses in higher plants (Adametal.1994; Logemannetal., 1995; Baerson etal., 1993). At present, there are many types of transcription factors found ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00
Inventor 唐晓艳周君莉陈竹锋刘东风
Owner FRONTIER LAB OF SYST CROP DESIGN CO LTD
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