Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same

A salivary protein and lectin technology, applied in the field of lectin chips based on protein detection of sugar chain markers, can solve the problem of affecting intercellular adhesion, damage to β-catenin and P120 proteins, and destruction of catenin and N-sugar chains Combining issues such as stability

Active Publication Date: 2016-06-08
深圳格道糖生物技术有限公司
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Problems solved by technology

[0006] For example, some functional sugar chains have been found in the research of gastric cancer, and the representative one is the up-regulated expression of N-acetylglucosaminyltransferase V (GnT-V) in gastric cancer cells, which leads to the synthesis of β1,6-linked N-acetyl Glucosamine branched N-glycan (β1,6GlcNAcbranchedN-Gl

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  • Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same
  • Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same
  • Lectin microarray for detecting carbohydrate chain marker based on sialoprotein and detection method of carbohydrate chain marker using same

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[0038] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0039]A total of 12 healthy volunteers were selected and did not take any drugs within a week. There were 64 diagnosed gastric cancer patients (21 cases of gastric cancer stage I, 18 cases of stage II, 20 cases of stage III, and 5 cases of stage IV). The age distribution is as follows: Table 1 shows.

[0040] Table 1

[0041]

[0042] Healthy volunteers were used as the basic control group, and confirmed gastric cancer patients were used as the sample group.

[0043] (1) Saliva collection:

[0044] The above-mentioned people gargle with normal saline three times between 9 and 10 o'clock after meals, and then quickly collect the whole saliva secreted naturally. Collect at least 1ml of saliva and put it on ice immediately, and add protease inhibitors to prevent protein degradation. , add 1 μL per milliliter of saliva;

[0045] (2...

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Abstract

The invention relates to a lectin microarray for detecting a carbohydrate chain marker based on sialoprotein and a detection method of the carbohydrate chain marker using the same. The lectin microarray can nondestructively detect the carbohydrate chain marker by distinguishing sialoprotein. The lectin microarray comprises a testing lectin probe group, wherein the testing lectin probe group comprises a vicia villosa lectin, an aleuria aurantia lectin, a pea lectin, a winged bean lectin-II an a winged bean lectin-I, and the winged bean lectin-II and the winged bean lectin-I are used as internal reference lectins. The lectin microarray has the advantages that the human body is not injured when the carbohydrate chain marker for detecting the carbohydrate chain marker is harmless to a human body, less samples are used, the detection speed is high and the sensitivity is high, and the lectin microarray can be applied to identification of stomach cancer.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a protein-based lectin chip for detecting sugar chain markers and a method thereof. Background technique [0002] Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, especially in East Asia, Eastern Europe, and South America due to the lack of significant early features. Currently commonly used diagnostic markers for gastric cancer include: CEA, CA19-9, CA72-4, CA125, AFP, etc., but all have limitations. Histopathological examination is still the gold standard for the diagnosis of gastric cancer at present. However, in view of the inherent defects of histological examination, such as destructive examination, dynamic detection, and sampling differences, it is important to seek non-invasive examination for early diagnosis of gastric cancer. The key to gastric cancer. [0003] With the progress of society and the development of ...

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Application Information

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IPC IPC(8): G01N33/574
CPCG01N33/57446G01N2800/06
Inventor 李铮舒健于汉杰刘夏薇杜昊骐
Owner 深圳格道糖生物技术有限公司
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