Enterobacter sp. strain and application thereof

A technology of Enterobacter and Enterobacter strains, applied in Enterobacter FL and its application fields, can solve the problems of uncompleted research on aerobic denitrifying bacteria and lack of research on aerobic denitrifying bacteria, and achieve good environmental protection benefits and ease dissolution The effect of different oxygen requirements and broad application prospects

Inactive Publication Date: 2016-06-15
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the research on this kind of aerobic denitrification bacteria has not been perfect so far, and the extensiveness of aerobic denitri...

Method used

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  • Enterobacter sp. strain and application thereof
  • Enterobacter sp. strain and application thereof
  • Enterobacter sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Screening of bacterial strains

[0027] The aerobic denitrifying bacteria provided by the invention are separated from activated sludge, and the activated sludge used comes from Chongqing Jiguanshi Sewage Plant A 2 / O process secondary sedimentation tank, strains are screened using the dilution plate streaking method, the specific operation is as follows:

[0028] 1) Inoculate 1 mL of sludge taken from the secondary sedimentation tank of the sewage plant into LB liquid medium for enrichment and culture for 12-24 hours.

[0029] 2) Preparation containing NO 3 - -N (nitrate nitrogen) selection medium I, inoculate 1 mL of the above-mentioned LB-enriched culture solution into selection medium I, and culture in a constant temperature shaking incubator at 30°C and 120 rpm for 48 hours.

[0030] 3) Take another selective medium I, and add 1.5-2.0% agar powder to it to make solid medium II; take 1 mL of the culture solution in step 2) and inoculate it on a plate containin...

Embodiment 2

[0045] Enterobacter sp. FL to NO 3 - -N degradation

[0046] Place Enterobacter sp. FL in KNO 3 In the artificial simulated wastewater as the only nitrogen source and trisodium citrate dihydrate as the only carbon source, the effect of the strain on NO 3 - -N, TN removal and NO in the reaction process 2 - -N (nitrite nitrogen), NH 4 + - Accumulation of N (ammonia nitrogen). The initial TOC (total organic carbon) concentration of the water distribution is about 800mg / L, the TN concentration is about 80mg / L, and the pH is controlled at 7.0-8.0. After inoculation, culture at constant temperature and shaking at 30°C and 120rpm for 72 hours, and take samples every 12 hours to determine relevant indicators. Depend on image 3 It can be seen that the strain can use trisodium citrate as an organic carbon source to realize NO 3 - - Efficient removal of N and TN, the removal rates can reach up to 85% and 70% respectively, and a small amount of NO is accumulated in the reacti...

Embodiment 3

[0048] Analysis of Extracellular Polymer Secretion Characteristics of Enterobacter sp. FL

[0049] To test the extracellular polymer secretion characteristics of Enterobactersp. FL, take the relationship between the extracellular polymer secretion of Enterobactersp. FL and bacterial cell growth and cell aggregation and self-sedimentation as an example. Enterobacter FL was continuously cultured in artificial simulated wastewater for 84 hours, and samples were taken every 12 hours to determine the OD 600 , cell dry weight and extracellular protein, polysaccharide content.

[0050] The water quality of the simulated wastewater is (per L): KNO 3 0.58g; 3.27g of trisodium citrate dihydrate; 4g of NaCl; 1g of yeast powder; 2g of beef extract;

[0051] The growth of Enterobacter FL in the culture medium and the secretion of extracellular polymers are as follows: Figure 4 shown. Depend on Figure 4 It can be seen that strain FL grows well under this culture condition, showing OD...

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Abstract

The invention discloses an Enterobacter sp. strain and application thereof. The strain is Enterobacter sp. FL which is collected by China General Microbiological Culture Collection Center (CGMCC) on January 12th, 2016; and the collection number is CGMCC No.11993. The Enterobacter sp. FL can be applied to the field of nitrogenous organic wastewater treatment, and can remove nitrate nitrogen in wastewater under aerobic conditions under the action of denitrification. The Enterobacter sp. secretes and generates extracellular polymers mainly composed of proteins, polysaccharides and the like in the denitrification process. The polymers can initiate spontaneous aggregation and precipitation of bacterial cells and implement bacterium-water separation. The strain has the aerobic denitrification capacity, can spontaneously aggregate to form a precipitate in the denitrification process, and has the characteristics of aggregation and spontaneous precipitation, so that a reactor using the strain as functional bacteria is established in the later period, thereby implementing aerobic denitrification and enabling the acquisition of clear effluent water by sludge-water separation. The strain has wide application prospects and favorable social and environmental benefits.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Enterobacter sp. FL and its application. Background technique [0002] With the rapid development of modern industry and social economy, the problems caused by environmental pollution are becoming more and more serious. Among them, water pollution has become one of the main problems of environmental pollution, and water eutrophication caused by nitrogen imbalance in water is an important cause of serious water pollution. Performance. The nitrogen balance in nature is gradually broken, not only causing serious environmental pollution and economic losses, but even threatening human health and ecological security. With the continuous development of sewage treatment technology, sewage denitrification technology has become increasingly mature, while biological denitrification is It is one of the more economical and effective methods in the currently recognized ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/16
CPCC02F3/02C02F3/34C02F2101/163C12N1/205C12R2001/07Y02W10/10
Inventor 赵彬汪霞田东伟田梦安强
Owner CHONGQING UNIV
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