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A Strain of Enterobacteriaceae and Its Application

A technology of Enterobacter and Enterobacter strains, applied in Enterobacter FL and its application fields, can solve the problems of uncompleted research on aerobic denitrifying bacteria and lack of research on aerobic denitrifying bacteria, and achieve good environmental protection benefits and ease dissolution The effect of different oxygen requirements and stable performance

Inactive Publication Date: 2019-06-28
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the research on this kind of aerobic denitrification bacteria has not been perfect so far, and the extensiveness of aerobic denitrification needs to be further determined.
Moreover, the research on aerobic denitrifying bacteria with flocculation and aggregation performance is even less

Method used

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  • A Strain of Enterobacteriaceae and Its Application
  • A Strain of Enterobacteriaceae and Its Application
  • A Strain of Enterobacteriaceae and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Screening of bacterial strains

[0027] The aerobic denitrifying bacteria provided by the invention are separated from activated sludge, and the activated sludge used comes from Chongqing Jiguanshi Sewage Plant A 2 / O process secondary sedimentation tank, strains are screened using the dilution plate streaking method, the specific operation is as follows:

[0028] 1) Inoculate 1 mL of sludge taken from the secondary sedimentation tank of the sewage plant into LB liquid medium for enrichment and culture for 12-24 hours.

[0029] 2) Preparation containing NO 3 - -N (nitrate nitrogen) selection medium I, inoculate 1 mL of the above-mentioned LB-enriched culture solution into selection medium I, and culture in a constant temperature shaking incubator at 30°C and 120 rpm for 48 hours.

[0030] 3) Take another selection medium I, and add 1.5-2.0% agar powder to it to make solid medium II; take 1 mL of the culture solution in step 2) and inoculate it on a plate containin...

Embodiment 2

[0045] Enterobacter sp. FL to NO 3 - -N degradation

[0046] Place Enterobacter sp. FL in KNO 3 In the artificial simulated wastewater as the only nitrogen source and trisodium citrate dihydrate as the only carbon source, the effect of the strain on NO 3 - -N, TN removal and NO in the reaction process 2 - -N (nitrite nitrogen), NH 4 + - Accumulation of N (ammonia nitrogen). The initial TOC (total organic carbon) concentration of the water distribution is about 800mg / L, the TN concentration is about 80mg / L, and the pH is controlled at 7.0-8.0. After inoculation, culture at constant temperature and shaking at 30°C and 120rpm for 72 hours, and take samples every 12 hours to determine relevant indicators. Depend on image 3 It can be seen that the strain can use trisodium citrate as an organic carbon source to realize NO 3 - - Efficient removal of N and TN, the removal rates can reach up to 85% and 70% respectively, and a small amount of NO is accumulated in the reacti...

Embodiment 3

[0048] Analysis of Extracellular Polymer Secretion Characteristics of Enterobacter sp. FL

[0049] To test the extracellular polymer secretion characteristics of Enterobacter sp. FL, take the relationship between the extracellular polymer secretion of Enterobacter sp. FL and bacterial cell growth, cell aggregation and self-sinking as an example. Enterobacter FL was continuously cultured in artificial simulated wastewater for 84 hours, and samples were taken every 12 hours to determine the OD 600 , cell dry weight and extracellular protein, polysaccharide content.

[0050] The water quality of the simulated wastewater is (per L): KNO 3 0.58g; 3.27g of trisodium citrate dihydrate; 4g of NaCl; 1g of yeast powder; 2g of beef extract;

[0051] The growth of Enterobacter FL in the culture medium and the secretion of extracellular polymers are as follows: Figure 4 shown. Depend on Figure 4 It can be seen that strain FL grows well under this culture condition, showing OD 600 A...

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Abstract

The invention discloses a strain of enterobacteriaceae and its application. The strain is Enterobacteriaceae ( Enterobacter sp.) FL, was deposited in the General Microorganism Center (CGMCC) of China Committee for the Collection of Microorganisms on January 12, 2016, with the deposit number CGMCC No.11993. The Enterobacter FL can be applied in the field of nitrogen-containing organic wastewater treatment, and removes nitrate nitrogen in the wastewater through denitrification under aerobic conditions; the Enterobacter FL secretes protein, polysaccharide, etc. as the main components during the denitrification process Extracellular polymers, which can trigger the spontaneous aggregation and sedimentation of bacterial cells, and realize the separation of bacteria and water. The strain has the ability of aerobic denitrification and denitrification, and can self-aggregate to form precipitates at the same time. It has the characteristics of self-sinking, so that the reactor with this strain as a functional bacterium can be constructed later to realize aerobic denitrification and mud-water separation. It is possible to obtain clarified effluent, which has broad application prospects and good social and environmental protection benefits.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a strain of Enterobacter sp. FL and its application. Background technique [0002] With the rapid development of modern industry and social economy, the problems caused by environmental pollution are becoming more and more serious. Among them, water pollution has become one of the main problems of environmental pollution, and water eutrophication caused by nitrogen imbalance in water is an important cause of serious water pollution. which performed. The nitrogen balance in nature is gradually broken, not only causing serious environmental pollution and economic losses, but even threatening human health and ecological security. With the continuous development of sewage treatment technology, sewage denitrification technology has become increasingly mature, while biological denitrification is It is one of the more economical and effective methods in the currently recogni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01C02F101/16
CPCC02F3/02C02F3/34C02F2101/163C12N1/205C12R2001/07Y02W10/10
Inventor 赵彬汪霞田东伟田梦安强
Owner CHONGQING UNIV
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