Microbial strain capable of remedying heavy metal-contaminated soil and application of microbial strain

A technology that pollutes soil and heavy metals, applied in the field of microorganisms, can solve the problems of soil destruction, high implementation cost, pollution, etc., and achieve the effect of small soil disturbance, high implementation cost and good effect

Active Publication Date: 2016-06-15
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention has certain limitations for existing physical and chemical methods for treati...

Method used

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  • Microbial strain capable of remedying heavy metal-contaminated soil and application of microbial strain
  • Microbial strain capable of remedying heavy metal-contaminated soil and application of microbial strain
  • Microbial strain capable of remedying heavy metal-contaminated soil and application of microbial strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the screening of microbial strain of the present invention

[0029] Liquid-phase enrichment method: Weigh an appropriate amount of cadmium-contaminated soil sample and add it to a conical flask filled with LB liquid medium, and add an appropriate amount of glass beads. Shake the triangular flask at 28-30° C., 150-170 rpm; transfer the soil mixture into a centrifuge tube, and take the supernatant after centrifugation as the source of cadmium-inactivated microorganisms. The above supernatant was inoculated into cadmium-containing LB liquid medium, and cultured with shaking at 28-30° C. and 150-170 rpm. Draw 1-2mL with a sterile pipette and transfer to another enrichment culture Erlenmeyer flask. After three transfers in this way, streak the plate on LB solid medium and inorganic salt solid medium respectively to isolate single colonies.

[0030] The supernatants were diluted 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 、10 -8 , absorb an appropria...

Embodiment 2

[0031] Embodiment 2: the identification of microbial strain of the present invention

[0032] The DNA of the strain was extracted according to the steps of the Tiangen bacteria genomic DNA extraction kit (TIANampbacteriaDNAkit) for identification of strains. Among the results with the highest matching degree, the measured 16SrDNA sequence of the strain has 100% homology with the 16SrDNA sequence of Burkholderia cepacia (Burkholderiacepacia), and it can be determined that the strain is Burkholderia cepacia. Burkholderia cepacia.

Embodiment 3

[0033] Embodiment 3: the cadmium passivation characteristic of bacterial strain of the present invention

[0034] The isolated pure culture strains were inoculated in LB liquid medium, and cultured by shaking at 28-30°C and 160-170rpm for 18-20h, in which a group of bacterial cells were first inactivated at 121°C for 15min, and then 6000- Centrifuge at 8000rpm at low temperature for 15-20min to collect dead bacteria as a dead cell inactivator; another group of bacteria cells are directly centrifuged at 3000-5000rpm at low temperature for 20-30min to collect live bacteria and wash them with sterile water 2-3 times. Acts as a passivating agent for living cells. Two groups of bacteria were added to cadmium-containing (50-300mg / LCd 2+ ) in an aqueous solution for adsorption experiments, the results are as follows figure 1 shown. according to figure 1 It can be seen from the results that both of them have strong ability to remove cadmium ions, but the adsorption capacity of liv...

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PUM

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Abstract

The invention relates to the field of microorganisms and discloses a microbial strain capable of remedying heavy metal-contaminated soil and application of the microbial strain. The preservation number of the microbial strain is CGMCC No.10843. A strain of burkholderia cepacia is screened from the soil contaminated by heavy metal cadmium and can purify the exchangeable cadmium in the soil and significantly reduce the content of the exchangeable cadmium. The microbial strain is extremely-strong in tolerance to the cadmium and small in soil disturbance, has a stronger biological remediation function, meanwhile can improve the activity of soil dehydrogenase and can also be applied to the remediation of the heavy metal-contaminated soil and the improvement of the activity of the soil dehydrogenase as well as the preparation of relevant products.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a microbial strain for repairing heavy metal polluted soil and its application. Background technique [0002] Due to long-term mining, metal smelting and application of phosphorus-containing pesticides, a large amount of heavy metal pollutants are enriched in the soil. Heavy metal polluted soil is concealed, cumulative and hysteresis, so it is not easy to attract attention. In recent years, the problem of soil pollution by heavy metals in my country has become increasingly prominent, posing a great threat to the soil quality of cultivated land and the health and safety of people's livelihood. According to the survey results of the national soil pollution situation by the Ministry of Environmental Protection and the Ministry of Land and Resources of China, the soil in my country is seriously polluted by heavy metals such as cadmium, arsenic, lead, chromium, and nickel. / 5, among whi...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 何声宝张威罗安娜刘楠王英元冯晓民
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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