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Oral anti-parasitic composition

A technology of antiparasitic drugs and compositions, which is applied in the direction of drug combinations, effective ingredients of hydroxyl compounds, anti-infective drugs, etc., and can solve problems such as skin damage and skin irritation

Active Publication Date: 2016-06-15
MARS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem with these spot-on methods is that the animal's skin can be irritated and can cause skin damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1: Proliferation assay of the N. caninum parasite in the presence of essential oils

[0081] Proliferation of the parasite in the cells was determined by calculating the specific radioactivity of added tritiated uracil and compared to a control without essential oil (100% proliferation). The assay is performed in 24-well plates and the entire assay lasts approximately 24 hours. A positive control was obtained using pyrimethamine (IC50: 0.1 μg / mL). image 3 The IC50 for each essential oil solution tested at different concentrations in the presence of N. caninum is shown.

[0082] Table 1 (below) outlines image 3 The result of embodiment 1 in. Values ​​indicate inhibition of proliferation. The dotted results show the IC50 for each essential oil tested.

[0083]

Embodiment 2

[0085] Toxoplasma gondii and Neospora caninum Inhibition of proliferation was determined by the addition of tritiated uracil, using the MTT assay Assess the viability of HFF cells. The results are shown in Figure 4 middle.

[0086] Figure 4 It was shown that various essential oils maintain cell viability HFF close to 80% at concentrations that have proliferation inhibitory properties against various parasites.

[0087] Insecticidally active oils could be observed with IC50 concentrations obtained for T. gondii and N. caninum. However, F oil and G oil had little or no effect on proliferation inhibition of N. caninum.

Embodiment 3

[0088] Example 3: Proliferation assay of Toxoplasma gondii in the presence of a combination of essential oils

[0089] The groups of essential oil combinations are: AB, AE, AD and BE.

[0090] A 3×3 factorial design was adopted for each combination. Theoretical IC50s were adjusted to the measured rates of inhibition of proliferation, which were then used as a basis for making dilutions and subsequently combining 9 different ratios of the 2 essential oils in the mixture.

[0091]

[0092] For each combination, 9 T. gondii proliferation inhibition assays were performed (all data not shown). Again, inhibition of proliferation was determined by addition of tritiated uracil.

[0093] Mixture BE (see Figure 5 ): shows that the mixture BE has a synergistic effect on the proliferation of Toxoplasma gondii.

[0094] Only 100 ppm of essential oil B corresponds to IC58 (58% inhibition of proliferation).

[0095] Only 75 ppm of essential oil E corresponds to IC88 (88% inhibitio...

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PUM

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Abstract

The present invention relates to a composition for use as an oral anti-parasitic, the composition comprises one or more of an essential oil which contains gamma-terpinene, and / or carvacrol, and / or thymol, and / or terpinenol, and / or eucalyptol and / or eugenol, an essential oil of genus Cinnamomum, Eugenia, Eucalyptus or a source of saponin. It also relates to a method of preventing or treating parasitic infection in an animal, the method comprising orally administering to said animal a composition comprising one or more of an essential oil which contains gamma-terpinene, and / or carvacrol, and / or thymol, and / or terpinenol, and / or eucalyptol and / or eugenol, an essential oil of genus Cinnamomum, Eugenia, Eucalyptus or a source of saponin.

Description

technical field [0001] The present invention relates to a composition used as an oral antiparasitic drug, the composition comprising one or more of the following: comprising gamma-terpinene (gamma-terpinene), and / or carvacrol (carvacrol) , and / or thymol (thymol), and / or terpineol (terpinenol), and / or eucalyptol (eucalyptol), and / or eugenol (eugenol) essential oil, camphor (Cinnamomum), Essential oils of Eugenia, Eucalyptus or sources of saponin. The invention also relates to a method of preventing or treating a parasitic infection in an animal, the method comprising orally administering to the animal a composition comprising one or more of the following: comprising gamma-terpine and / or carvacrol, and / or thymol, and / or terpineol, and / or eucalyptol, and / or eugenol, of the genus Cinnamomum genus, Cinnamon genus, Eucalyptus Essential oil or saponin source. Background technique [0002] Parasitic infections can infect all animals. In particular, dogs and cats are the main hos...

Claims

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Application Information

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IPC IPC(8): A61K36/23A61K36/53A61K36/54A61K36/61A61K31/7048A61K31/045A61K31/05A61P33/02A23L33/10
CPCA61K31/7034A61K36/23A61K36/53A61K36/54A61K9/0053A61K36/61A61P33/00A61P33/02A61P33/10Y02A50/30A61K2300/00A61K33/02A61K36/36A61K31/704A61K47/36
Inventor A·弗吉耶N·勒鲁克塞尔
Owner MARS INC
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