Flat-field correction method based on line-scan digital camera scanning

Abstract

The present invention discloses a flat-field correction method based on line-scan digital camera scanning. The method is applied to a line-scan digital camera and comprises the following steps of S1 obtaining a preview image of a pathological section, focusing the line-scan digital camera according to the preview image, and obtaining a Z-axis focal length position of the line-scan digital camera; S2 using the line-scan digital camera to scan the pathological section by taking the Z-axis focal length position as reference to obtain a pathological section image; S3 separating the pathological section image into three grey-scale maps according to red, green and blue three color channels, and calculating to obtain the correction coefficient curves of the colors respectively according to the three grey-scale maps, and carrying out the flat-field correction on the image scanned by the line-scan digital camera according to the correction coefficient curves of the three colors. The flat-field correction method based on the line-scan digital camera scanning of the present invention can filter the interference of the dust, the impurity and the own noise of the line-scan digital camera, and achieves the purpose of improving the flat-field correction accuracy.

Description

technical field [0001] The invention relates to the field of microscopic technology, in particular to a flat field correction method based on line array camera scanning. Background technique [0002] In the era of rapid development of intelligent digitalization, the utilization rate of line scan cameras continues to increase. At present, line scan cameras are mainly used in industries such as machine vision, industrial inspection, and pathological slide scanning. The line-scan camera used in the pathological slice digital scanner system is used to scan and collect images of traditional pathological chromosomal pathological slices. Through the computer connected to the instrument, the images can be browsed, saved, diagnosed and remote consultation. However, due to the inconsistency of the photosensitive coefficient of each point of the photosensitive element of the line scan camera and the uneven brightness of the optical path, the gray value of the image is uneven, resultin...

AI Technical Summary

Problems solved by technology

During the flat-field correction process of the line array camera, if dust and impurities appear in the correction data, the correction data will be affected, so that the purpose of flat-field correction cannot be achieved; Ensure that the focal length position is free from dust and impurities, or that there is no error between the focal length position and the scanned focal length position, so the results of flat-field correction are prone to unsatisfactory situations

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