Unlock instant, AI-driven research and patent intelligence for your innovation.

Compositions and methods for multiplex analysis of NRAS and BRAF nucleic acids

A technique for composition and nucleic acid samples, applied in the direction of biochemical equipment and methods, microbial determination/testing, etc., can solve the problem of low multiplicity

Inactive Publication Date: 2016-06-22
QIAGEN MANSFIELD
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional methods used to detect such mutations provide a lower multiplex capability than is required for comprehensive clinical diagnosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compositions and methods for multiplex analysis of NRAS and BRAF nucleic acids
  • Compositions and methods for multiplex analysis of NRAS and BRAF nucleic acids
  • Compositions and methods for multiplex analysis of NRAS and BRAF nucleic acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0155] This article describes the development of an NRAS / BRAF point mutation analysis panel that can be used, for example, in ICEPlex TM A multiplex PCR assay using nucleic acid samples to detect the 12 clinically important NRAS mutations and the other 4 BRAF mutations in a single reaction on the system or similar.

[0156] The RAS gene is a proto-oncogene frequently mutated in human cancers, encoded by three ubiquitously expressed genes HRAS, KRAS and NRAS. These RAS genes have GTP / GDP binding activity and GTPase activity, and their proteins can participate in the control of cell growth. RAS proteins exhibit isoform-specific functions; and in NRAS, genetic mutations that alter amino acid residues 12, 13, or 61 activate the encoded protein's potential to transform cultured cells, which is associated with multiple It is associated with a variety of human neoplasms, especially cancers of the skin, blood and lymphoid tissues.

[0157] NRAS / BRAF point mutation analysis panel det...

Embodiment 2

[0177] Table 4: Another NRAS primer set compatible with the conditions described in Example 1 (including the BRAF primers shown in Table 1)

[0178]

[0179]

[0180] The primers in Table 4 are compatible with both cDNA templates and genomic DNA.

[0181]

[0182]

[0183]

[0184]

[0185]

[0186]

[0187]

[0188]

[0189]

[0190]

[0191]

[0192]

[0193]

[0194]

[0195]

[0196]

[0197]

[0198]

[0199]

[0200]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses compositions and methods for multiplex analysis of NRAS and bRAF nucleic acids. Described herein are methods and assays relating to the detection of NRAS and / or BRAF alterations (e.g. variations in copy number and expression level, and / or the presence of mutations, including point mutations). Existing methods are limited in their clinical usefulness by, e.g., limited sensitivity, inter-lab discordance, or inability to provide the necessary multiplex ability. The methods and assays provided herein permit multimodal, multiplex assaying for faster, more cost-effective testing and screening of patients, permitting improved healthcare.

Description

[0001] Cross References to Related Applications [0002] Pursuant to 35 U.S.C. §119(e), this application claims priority to U.S. Provisional Application No. 61 / 865,754, filed August 14, 2013, the contents of which are hereby incorporated by reference in their entirety. [0003] sequence listing [0004] This application contains a Sequence Listing, which has been filed electronically in ASCII format, and is hereby incorporated by reference in its entirety. Said ASCII copy was created on July 29, 2014, named 046264-078891-PCT_SL.txt, and was 28,830 bytes in size. technical field [0005] The technology described herein relates to assays and methods that enable the detection of the presence and / or absence of mutations, including point mutations. Background technique [0006] The development of personalized medicine has enabled the identification of genes that, when mutated and altered, can cause disease. For example, a sequence encoding a gene may be mutated in a subject wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/156C12Q2600/16C12Q1/6886C12Q2563/107C12Q2527/143
Inventor 利利·I·贡希兰·马登纳哈利·迪瓦卡
Owner QIAGEN MANSFIELD