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Method for thawing gametes or embryos on frozen carriers and special dishes for vitrification and thawing

A freezing carrier and vitrification technology, applied in the medical field, can solve the problems of affecting the developmental potential of gametes or embryos and pregnancy outcomes, the inability to achieve rapid operation, and the effects of gamete or embryo thawing effects, to improve developmental potential, good practicability, The effect of improving pregnancy outcomes

Inactive Publication Date: 2018-05-22
戴志俊 +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, when thawing gametes or embryos on frozen carriers, more thawing liquid is required in the petri dish, and the microscope field of view needs to be adjusted in time during the thawing operation, which not only cannot achieve the purpose of fast operation, but also easily affects the The thawing effect has a certain impact, which ultimately affects the developmental potential of gametes or embryos and pregnancy outcomes

Method used

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  • Method for thawing gametes or embryos on frozen carriers and special dishes for vitrification and thawing
  • Method for thawing gametes or embryos on frozen carriers and special dishes for vitrification and thawing

Examples

Experimental program
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Effect test

Embodiment 1

[0024] See figure 1 and figure 2 Shown: Vitrification and thawing special dishes, used for thawing gametes or embryos on frozen carriers.

[0025] Specifically, the special dish includes a dish plate 10 and a dish cover (not shown in the figure). The dish 10 is provided with a thawing liquid hole 20 with an upper opening, and the dish cover is used to cover the thawing liquid hole 20 at ordinary times. Seen from top to bottom, the thawing liquid hole 20 is an isosceles triangle, preferably an equilateral triangle. Viewed from the side, the thawing liquid hole 20 is a right-angled trapezoid with a smaller lower side and a straight side on the right. That is, the right angle of this right-angled trapezoid is located at the lower right corner. Therefore, the left side wall 21 of the thawing liquid hole 20 is a slope, and the bottom 22 is a flat bottom. In this way, when the gametes or embryos on the frozen carrier are thawed, less thawing liquid is put into it, which helps ...

Embodiment 2

[0027] See figure 1 and figure 2 Shown: Method for thawing gametes or embryos on cryocarriers, including the following steps:

[0028] (1) Prepare a special dish for vitrification and thawing. The special dish for vitrification and thawing is the special dish described in Example 1.

[0029] (2) Add thawing liquid into the thawing liquid hole 20 of the vitrification and thawing special dish.

[0030] (3) Take out the gametes or embryos from the liquid nitrogen by using the freezing rod 30, and the gametes or embryos are located on the blade 31 of the freezing rod 30, and make the blade 31 obliquely along the left side wall 21 of the thawing liquid hole 20. Moving downward, the blade 31 is inserted into the bottom 22 of the thawing liquid hole 20 and submerged in the thawing liquid.

[0031] (4) After using a microscope to observe that the gametes or embryos fall into the thawing solution hole 20 , the freezing rod 30 is removed, and the gametes or embryos are kept in equi...

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Abstract

The invention discloses a method for unfreezing a gamete or an embryo on a frozen carrier.The method sequentially includes the following steps that a vessel special for vitrification unfreezing is prepared, a vessel disc of the vessel special for vitrification unfreezing is provided with an unfreezing liquid hole with the upper portion being open, the left side wall of the unfreezing liquid hole is an inclined plane, and the bottom of the unfreezing liquid hole is a flat bottom; unfreezing liquid is added into the unfreezing liquid hole; a frozen carrying pole is adopted to take the gamete or the embryo out of liquid nitrogen, the gamete or the embryo is located on a blade of the frozen carrying pole, and the blade is made to downwards move in an inclined mode along the left side wall of the unfreezing liquid hole till the blade is soaked in the unfreezing liquid; a frozen carrying pole is utilized to observe that the gamete or the embryo falls.The method has the advantages that the gamete or the embryo on the frozen carrier can be unfrozen rapidly and accurately, so that developmental potentiality and pregnancy outcome of the gamete or the embryo are improved.The invention further discloses the vessel special for vitrification unfreezing.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for thawing gametes or embryos on a freezing carrier. The present invention also relates to a special vitrified thawing dish used in the gamete or embryo thawing method on the freezing carrier. Background technique [0002] At present, when thawing gametes or embryos on frozen carriers, more thawing liquid is required in the petri dish, and the microscope field of view needs to be adjusted in time during the thawing operation, which not only cannot achieve the purpose of fast operation, but also easily affects the The thawing effect has a certain impact, which ultimately affects the developmental potential of gametes or embryos and pregnancy outcomes. Contents of the invention [0003] The object of the present invention is to provide a method for thawing gametes or embryos on a frozen carrier, which has the characteristics of being able to quickly and accurately tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A01N1/02
CPCA01N1/0284
Inventor 戴志俊季钢
Owner 戴志俊
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