Stem cell preparation for repairing skin ulcer

A technology of stem cell preparation and skin repair, which is applied in the field of cell engineering, can solve the problems of poor skin ulcer effect, and achieve the effect of fast and effective skin repair, skin repair and increased proliferation

Inactive Publication Date: 2016-06-29
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a stem cell preparation for repairing skin ul...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The stem cell preparation for repairing skin ulcer provided by the present invention includes the following components; and in the cell preparation, the concentration of each component is as follows:

[0049] Mesenchymal stem cells 5×10 7 a / ml;

[0050] Platelet plasma lysate 15%;

[0051] Human serum protein 40mg / ml;

[0052] Sodium citrate adjusts the injection to PH=5.5;

[0053] Sodium chloride 0.2mol / L;

[0054] Fasudil 10μmol / L;

[0055] Doxycycline 8U / mL.

[0056] Wherein, the source process of mesenchymal stem cells includes the following steps:

[0057] S1a, obtaining the umbilical cord;

[0058] The umbilical cord of the full-term cesarean section fetus was about 4-6 cm long, and the cord blood was drawn under sterile conditions, stored in PBS at 4°C, and processed within 4 hours.

[0059] S2a, separation and purification

[0060] Put the umbilical cord about 4-6cm long into the ultra-clean bench, remove the arteriovenous and adventitia, PBS (wherein,...

Embodiment 2

[0067] The stem cell preparation provided in this example for repairing skin ulcers includes the following components, and the concentration of each component in the cell preparation is as follows:

[0068] Mesenchymal stem cells 2×10 7a / ml;

[0069] Platelet plasma lysate 5%;

[0070] Human serum protein 20mg / ml;

[0071] Sodium citrate adjusts the pH of the injection to 5.0;

[0072] Sodium chloride 0.1mol / L;

[0073] Fasudil 5 μmol / L;

[0074] Doxycycline 1U / mL.

[0075] Wherein, the source process of mesenchymal stem cells and platelet plasma lysate is basically the same as that of Example 1.

Embodiment 3

[0077] The stem cell preparation provided in this example for repairing skin ulcers includes the following components, and the concentration of each component in the cell preparation is as follows:

[0078] Mesenchymal stem cells 8×10 7 a / ml;

[0079] Platelet plasma lysate 20%;

[0080] Human serum protein 80mg / ml;

[0081] Sodium citrate adjusts the pH of the injection to 6.0;

[0082] Sodium chloride 0.5mol / L;

[0083] Fasudil 10μmol / L;

[0084] Doxycycline 15U / mL.

[0085] Wherein, the source process of mesenchymal stem cells and platelet plasma lysate is basically the same as that of Example 1.

[0086] In order to further verify the remarkable effect of the stem cell preparation provided by the present invention for repairing skin ulcers, the following experiments and experimental data are used for specific illustration.

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PUM

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Abstract

The invention relates to a stem cell preparation for repairing skin ulcer. The stem cell preparation consists of mesenchymal stem cells and exogenous cell factors. The stem cell preparation is prepared from the mesenchymal stem cells in combination with the exogenous cell factors, so that the directional differentiation of the mesenchymal stem cells towards endothelial cells is promoted; and through the paracrine of the mesenchymal stem cells as well as the exogenous cell factors, the growth of the endothelial cells is stimulated, fibroblast and capillary growth as well as collagen synthesis and granulation in a wound surface are enhanced, and the proliferation of skin keratinocytes and endothelial cells is improved, so that the purpose of rapidly and effectively repairing skin with ulcer injury is achieved.

Description

technical field [0001] The invention relates to the technical field of cell engineering, in particular to a stem cell preparation for repairing skin ulcers. Background technique [0002] Chronic skin ulcer, also known as refractory skin ulcer, is a common refractory disease with complex etiology, long course of disease and recurrent attacks, which can cause skin epidermis, part of the dermis and even subcutaneous fat defects. Pathogenic factors include diabetes, trauma, compression and infection, among which diabetes is the main reason. At present, there are about 92 million diabetic patients in my country, and 15% to 25% of patients will develop foot ulcers during their disease process. [0003] At present, there is no guideline for the treatment of chronic skin ulcers. Clinically, debridement, negative pressure therapy, anti-infective drugs and dressings are mostly used. These methods cannot achieve the effect of promoting wound healing and protecting the wound surface, a...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P17/02A61K35/17A61K31/551A61K31/65A61K38/38A61K33/14A61K31/194
CPCA61K38/30A61K31/194A61K31/551A61K31/65A61K33/14A61K35/28A61K38/046A61K38/1808A61K38/1825A61K38/1841A61K38/1858A61K38/1866A61K38/385A61K2300/00
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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