Method for tomato spotted wilf virus inoculation

A tomato spotted wilt virus and tomato technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the time-consuming and labor-consuming problems of tomato spotted wilt virus, and achieve the effect of increasing speed and speeding up breeding

Inactive Publication Date: 2016-07-06
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the time-consuming and labor-consuming problem of manual inoculation of tomato spotted wilt virus, and provide a method for inoculating tomato spotted wilt virus with high inoculation efficiency and rate

Method used

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Embodiment Construction

[0013] The method for tomato spotted wilt virus inoculation of the present embodiment, its concrete steps are as follows:

[0014] Step 1: Prepare 50mM pH7.2 phosphate buffer, sterilize at 121°C for 20 minutes, and set aside;

[0015] The second step: sow tomato, and carry out tomato spotted wilt virus inoculation when the seedling grows 4 true leaves;

[0016] Step 3: Take out the tomato leaves infected with tomato spotted wilt virus stored at -80°C, weigh them, cut them into pieces with scissors, put them in a mortar, add phosphate buffer solution at a ratio of mass:volume=1:5, and grind until Homogenized;

[0017] Step 4: Put the ground homogenate into a centrifuge tube, centrifuge at 5000rpm at 4°C for 10 minutes, take the supernatant and put it into a small plastic watering can;

[0018] Step 5: Take the tomato seedlings, immerse the whole tomato in the phosphate buffer solution in the ultrasonic cleaner, vibrate at 200W for 10 seconds, take it out, and spray the supern...

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Abstract

The invention relates to a method for inoculating tomato spotted wilt virus, which belongs to the field of plant protection. The method comprises the following steps: (1) configuring 50mM pH7.2 phosphate buffer solution and sterilizing; (2) sowing tomatoes, and growing to 4 true leaves for inoculation; (3) by 1:5 (mass:volume) Proportionally add phosphate buffer solution to grind the susceptible tomato leaves preserved at low temperature to homogenate; (4) take the homogenate and centrifuge at low temperature and high speed, and add the supernatant to a small plastic watering can; (5) immerse the tomato seedlings in the phosphate buffer in the ultrasonic cleaner After oscillating in the liquid for 10 seconds, the virus liquid in the watering can was sprayed onto the treated tomato seedling leaves; (6) after cultivating for 12 hours in a dark artificial climate box at 100% relative humidity, take it out and place it under conventional conditions nourish. The beneficial effect of the invention is that it can increase the virus inoculation speed of tomato seedlings, make the whole tomato leaves susceptible to the disease, quickly and efficiently identify the resistance of the tomato to the virus, and accelerate the selection of disease-resistant varieties.

Description

Technical field: [0001] The invention relates to a method for inoculating tomato spotted wilt virus, which belongs to the field of plant protection. Background technique: [0002] In recent years, tomato spotted wilt virus has become an important disease that endangers tomato production. Identifying and breeding resistant varieties is an effective way to control tomato spotted wilt virus. Field thrips transmission and laboratory inoculation are two commonly used methods for tomato cultivars infected with spotted wilt virus. Due to the growth characteristics of tomato, the living habits of thrips and the external environment, there are time and season limitations in the field natural disease identification of tomato resistance. Artificial inoculation in the laboratory can infect tomato seedlings all year round, thereby speeding up the process of disease-resistant breeding. [0003] Mechanical friction is the commonly used manual inoculation method at present. However, after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06
CPCA01G7/06
Inventor 赵凯朱海山张宏邓明华杨正安莫云容张培欣汤晓倩
Owner YUNNAN AGRICULTURAL UNIVERSITY
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