A kind of elispot detection kit for detecting bovine brucellosis

A detection kit, the technology of Brucella bovine, applied in the field of diagnosis of bovine brucellosis, the field of ELISPOT detection kit for detecting bovine brucellosis, can solve the problem that cannot satisfy the specific detection of bovine Brucella infection , The specificity is not ideal, and it cannot meet the problems of early detection of Brucella bovine infection, etc., and achieve the effect of good specificity, elimination of false positive interference, and strong affinity
CN105759054BActive Publication Date: 2017-10-20YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2017-10-20

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Abstract

The invention provides an ELISPOT detection kit for detecting bovine brucellosis, the ELISPOT detection kit includes: a support medium, a capture antibody, a detection antibody, a negative control and a positive control, and a specific stimulating agent, wherein the capture The antibody is the first bovine gamma interferon monoclonal antibody, the detection antibody is the second labeled bovine interferon gamma monoclonal antibody, and the specific stimulator is brucellin Br-PPD, wherein the support medium and reagent There is a microporous filter membrane on the contact surface; the capture antibody is coated on the support medium or the capture antibody and the support medium are placed separately; and the capture antibody and the detection antibody can combine with the bovine gamma interferon for antigen-antibody binding. The monoclonal antibody secreted by the hybridoma cell line used in the kit of the present invention has the advantages of high titer, good specificity, and strong affinity with natural antigens; compared with the Brucella antibody ELISA detection kit, it can realize the The early detection of Brucella infection excludes the interference of false positives caused by cross-reactions and shows good specificity.
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Description

technical field

[0001] The invention relates to a detection kit, in particular to an ELISPOT detection kit for detecting bovine brucellosis and its application in the field of bovine brucellosis diagnosis. Background technique

[0002] Bovine brucellosis (hereinafter referred to as "bull brucellosis") is a zoonotic chronic infectious disease caused by Brucella. After infection, livestock will have symptoms such as infertility and abortion, and bulls will have arthritis and orchitis. The ability to breed has declined significantly, seriously threatening the health of the herd, and causing great harm to animal husbandry production and human health.

[0003] Brucella is a facultative intracellular parasite that induces a cellular immune response after infection. The cellular immune response can produce a series of cytokines, which play an important role in the body's defense against infection. When the body is infected with Brucella, specific immunogenic proteins can activate...

Claims

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