Citicoline sodium medicine composition and pharmaceutical application of citicoline sodium medicine composition to preventing and treating ovarian cancer

A technology of citicoline sodium and composition, applied in the field of pharmaceutical composition of citicoline sodium, can solve the problem that affects the overall effect of chemotherapy and has not yet been seen with citicoline sodium and its pharmaceutical composition and ovarian cancer prevention and treatment. Relevant reports and other issues, to achieve the effect of highlighting the substantive characteristics, improving the control effect, and making significant progress

Inactive Publication Date: 2016-07-20
钱浩
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the clinical treatment methods for ovarian cancer are tumor cytoreductive surgery, chemotherapy and radiotherapy. According to the patient's condition, chemotherapy can be given before and after surgery. Therefore, chemotherapy plays an important role in the treatment of patients with advanced ovarian cancer. However, due to the drug resistance of tumor cells

Method used

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  • Citicoline sodium medicine composition and pharmaceutical application of citicoline sodium medicine composition to preventing and treating ovarian cancer
  • Citicoline sodium medicine composition and pharmaceutical application of citicoline sodium medicine composition to preventing and treating ovarian cancer
  • Citicoline sodium medicine composition and pharmaceutical application of citicoline sodium medicine composition to preventing and treating ovarian cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0021] Separation method: (a) Grind the dried root (2kg) of Polygala, extract with 80% ethanol under heat reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether (3L×3 times) 3 times), ethyl acetate (3L × 3 times) and water-saturated n-butanol (3L × 3 times) were extracted to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) step (a) Use D101 type macroporous resin to remove impurities from the n-butanol extract in (a), first elute with 10% ethanol for 8 column volumes, then use 70% ethanol for 10 column volumes, collect 70% eluate, and depressurize Concentrate to get 70% ethanol elution concentrate; (c) in step (b), 70% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 60:1 (10 column volumes), 30:1 ( 8 column volumes), 15:1 (10 column volumes) and ...

Embodiment 2

[0024] Embodiment 2: Prevention and treatment effect on ovarian cancer model

[0025] 1. Materials and Instruments

[0026] The human ovarian serous papillary cystadenocarcinoma cell line SKOV3 was provided by the Medical Experimental Center of Lanzhou University. Common RPMI-1640 medium (10% fetal bovine serum) culture. Citicoline sodium was purchased from China Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. RPMI-1640 culture medium, tetramethylazoblue (MTT), dimethyl sulfoxide (DMSO), L-glutamine Kehao Bioengineering Co., Ltd. Trypsin was purchased from Sigma, USA. Fetal bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd. Sodium dodecyl sulfonate (SDS) was purchased from Xi'an Zhouding Biotechnology Co., Ltd. HER2 (FITC marker) was purchased from Beijing Boaosen Biotechnology Co., Ltd. Acidified HER2 (FITC-labeled) was purchased from Beij...

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Abstract

The invention discloses a citicoline sodium medicine composition and pharmaceutical application of the citicoline sodium medicine composition to preventing and treating ovarian cancer. The citicoline sodium medicine composition contains citicoline sodium and a natural product compound (I) which is separated from dried roots of radix polygalae and is of a novel structure. Only common effects of preventing and treating the ovarian cancer can be realized when the citicoline sodium and the natural product compound (I) are individually used, but effects of preventing and treating the ovarian cancer can be obviously improved when the citicoline sodium and the natural product compound (I) are used with each other, and accordingly the citicoline sodium medicine composition can be developed to obtain medicines for preventing and treating the ovarian cancer. Compared with the prior art, the citicoline sodium medicine composition and the pharmaceutical application have the advantages of outstanding substantial characteristics and obvious advancement.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of citicoline sodium, in particular to a pharmaceutical composition of citicoline sodium and its medical application for preventing and treating ovarian cancer. Background technique [0002] Citicoline sodium is used for disturbance of consciousness after acute craniocerebral trauma and brain surgery. It can gradually restore the function of limbs to hemiplegia caused by cerebral apoplexy. It can also be used for function and consciousness disturbance caused by other acute injuries of the central nervous system. in ischemic cerebrovascular disease and vascular dementia. [0003] Citicoline sodium can promote brain substance metabolism and improve cerebral circulation by reducing cerebrovascular resistance and increasing cerebral blood flow. It can also enhance the function of the ascending reticular activation system of the brain stem, enhance the function of the verteb...

Claims

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Application Information

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IPC IPC(8): C07D307/92A61K31/343A61P35/00A61P15/00
CPCC07D307/92
Inventor 不公告发明人
Owner 钱浩
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