Deodorant organic compost fermentation inoculant and application thereof

A technology of organic composting and bacterial agents, applied in the direction of organic fertilizers, applications, bacteria, etc., can solve the problems of cumbersome steps, waste of materials and labor, and increased labor costs, and achieve the effects of improving the atmospheric environment, improving the working environment, and shortening the production cycle.

Inactive Publication Date: 2016-07-20
福建美家园生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method also has the following disadvantages: (1) it does not solve the dehydration step that accounts for a large proportion in the sludge treatment cost, and manual dehydration is still required, and the cost of reducing the moisture content to 50% is too high; (2) in sludge Adding zeolite to the sludge increases the impurity content of the fertilizer in the later stage of the sludge (3) The spray liquid needs to be sprayed for 0.5-1h, which is laborious and laborious
(4) The method steps are loaded down with trivial details, additionally increase labor cost
But this method also has the following disadvantages: (1) Trichoderma viride is present in the strains provided by this method, and its optimum growth temperature is 25-28° C., and compost needs to reach a compost temperature of more than 55° C. and keep it for 3 days to achieve harmlessness Standard, so high temperature may inactivate the bacteria
(2) The bacterial classification provided by this method contains lactic acid bacteria, and it is necessary to isolate the air when making lactic acid bacteria koji culture, which is nothing more than increasing the production cost for the process

Method used

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  • Deodorant organic compost fermentation inoculant and application thereof
  • Deodorant organic compost fermentation inoculant and application thereof
  • Deodorant organic compost fermentation inoculant and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0034] (1) Preparation of pure strain suspension for production:

[0035] (1-1) Use PDA medium to culture on a shaker at an ambient temperature of 29° C. to 35° C. (for example, 29, 30, 31, 32, 33, 34 or 35° C.) for 24 hours to obtain pure species of Pichia pastoris for production bacterial suspension; and / or

[0036] (1-2) Use liquid beef extract peptone medium to cultivate Pseudomonas at an ambient temperature of 29°C to 35°C (for example, 29, 30, 31, 32, 33, 34 or 35°C) and culture on a shaker for 24 hours , to obtain pure strain suspension for Pseudomonas production; and / or

[0037] (1-3) Cultivate on PDA medium at an ambient temperature of 25 to 30°C (for example, 25, 26, 27, 28, 29 or 30), for example 36 hours, to obtain a pure strain suspension of Trichoderma viride;

[0038] (1-4) in Na 2 S 2 o 3 Cultivate on an inorganic salt medium at an ambient temperature of 29-35°C (for example, 29, 30, 31, 32, 33, 34 or 35°C), for example, for 72 hours to prepare a pure bact...

Embodiment 1

[0087] Embodiment 1 is carried out in the following manner:

[0088] (a) Pseudomonas was cultivated at 30°C ambient temperature for 24 hours using liquid beef extract peptone medium, Pichia pastoris and Rhizopus oryzae were cultivated at 30°C ambient temperature on PDA medium for 36 hours, and Thiobacillus naples was cultivated at 30°C ambient temperature on PDA medium. Na 2 S 2 o 3 Cultivate for 72 hours at an ambient temperature of 30° C. on an inorganic salt medium to obtain a pure strain suspension for production.

[0089] (b) Inoculate the pure strain suspension into the bran culture medium at a ratio of 5% to 10%, culture it at a temperature of 30°C for 48-72 hours, check the solid fermented matter separately, and the number of viable bacteria reaches ≥ At 1 billion cells / g, the culture was terminated. After drying at low temperature (25°C), the solid bacterial agents of Pichia pastoris, Pseudomonas, Rhizopus oryzae and Thiobacillus naples are used for production. T...

Embodiment 2

[0099] Carried out in substantially the same manner as in Example 1, except that the Pichia pastoris (Pichia pastoris) inoculum and Rhizopus oryzae (Rhizopusoryzae) inoculum were used in a mass ratio of 1:1.

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PUM

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Abstract

The invention discloses a multi-strain compound inoculant for deodorization in fermentation of organic compost, a preparation method of the multi-strain compound inoculant and application of the multi-strain compound inoculant in deodorizing materials for organic compost. The multi-strain compound inoculant comprises or is composed of a Pichia pastoris inoculant, a pseudomonas inoculant, a Rhizopus oryzae inoculant and a Thiobacillus neapolitanus inoculant. The method includes the first step of preparing pure inoculum suspension for production, the second step of preparing solid inoculants for production and the third step of preparing the multi-strain compound inoculant. On the basis of common fermentation inoculants, it can be guaranteed that livestock manure does not emit many odorous substances in the fermentation process, the atmospheric environment and the working environment of a plant can be improved, the fertility of compost can be improved, and the production cycle can be shortened.

Description

technical field [0001] The invention relates to a technology for harmless treatment of organic waste, in particular to a deodorizing organic compost fermentation bacterial agent and its application. Background technique [0002] Composting is an ideal method for treating organic waste such as livestock manure and domestic sludge. Judging from the results of general composting, it has a good deodorizing effect on the materials after composting, but during the composting process, due to the increase in temperature, the decomposition of organic matter, etc., the materials in fermentation will be worse than those without treatment. The fresh raw material emits a more serious odor, causing air pollution, affecting the normal life of the surrounding residents, and even causing diseases. [0003] NH in the gas released by pig manure composting 3 、H 2 Sulfur and nitrogen-containing compounds such as S are the main odor-causing substances. At present, there are two main methods f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C05F15/00C12R1/84C12R1/38C12R1/845C12R1/01
CPCC12N1/20C05F3/00C05F11/08C12N1/14C12N1/16C05F11/00
Inventor 陈五岭郭彦钊
Owner 福建美家园生物科技股份有限公司
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