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Trichoderma sp. chlamydospore microcapsule and its preparation method and use

A technology of microcapsules and chlamydospores, applied in the field of agricultural biology, can solve the problems of short shelf life, little research, and difficulty in large-scale production, achieve strong viability and stress resistance, solve short shelf life, Multifunctional effect

Active Publication Date: 2016-07-27
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These preparations all have unstable spore storage and short shelf life. The storage time of live bacteria at room temperature will not exceed 3 months. At the same time, because the conidia are obtained by solid-state fermentation, it is difficult to produce them on a large scale, so the Trichoderma preparations currently on the market cannot completely replace chemical pesticides in plant disease control.
At present, domestic and foreign research on Trichoderma chlamydospore mainly focuses on its production conditions and shake flask culture optimization, and there are few studies on the industrial liquid fermentation and preparation of Trichoderma chlamydospore

Method used

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  • Trichoderma sp. chlamydospore microcapsule and its preparation method and use
  • Trichoderma sp. chlamydospore microcapsule and its preparation method and use
  • Trichoderma sp. chlamydospore microcapsule and its preparation method and use

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Embodiment

[0046] Preparation of Trichoderma Chlamydospores by Fermentation

[0047] Trichoderma was fermented in a 10000L fermenter to prepare Trichoderma chlamydospores, and the strain used was Trichoderma T4. Bacterial strains are isolated from plant rhizosphere soil and can be purchased from the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology (No. NY-T4). T4 solid fermentation process and research on the stability of its preparation", 2008, Vol.25, No.12, pages 52-56).

[0048] Fermentation medium: glucose 0.8%, corn flour 4%, molasses 5%, wheat bran 0.5%, soybean flour 3.2%, pH6.5-7.0.

[0049] Culture conditions: temperature 30°C, seed inoculation amount 6%, seed concentration 1×10 6 per mL, aeration ratio 1:2, tank pressure 0.04MPa, fermentation time 168 hours.

[0050] Fermentation is in the logarithmic phase for about 20-72 hours, the fermentation broth also begins to become viscous, and mycelium grows in large quantities. 72...

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Abstract

The invention discloses a trichoderma sp. chlamydospore microcapsule for preventing and treating plant diseases. The trichoderma sp. chlamydospore microcapsule comprises trichoderma sp. chlamydospores, a binder and a protective agent. The preparation method comprises 1, providing a trichoderma sp. chlamydospore fermentation liquor and 2, mixing the trichoderma sp. chlamydospore fermentation liquor, the binder and the protective agent and carrying out spray drying to obtain the microcapsule preparation. The trichoderma sp. chlamydospore microcapsule can be used for preventing and treating plant soil borne diseases and especially has effects of preventing and treating crucifer clubroot and grape gray mold rot. Compared with a conidiospore preparation produced by the traditional method, the trichoderma sp. chlamydospore microcapsule has strong vitality and adverse resistance, has more functions and is suitable for production and application. The trichoderma sp. chlamydospore microcapsule solves the problems of a short shelf life of the existing trichoderma sp. biopesticide, large scale production infeasibility of the existing trichoderma sp. biopesticide and dust in application and production.

Description

technical field [0001] The application relates to the field of agricultural biotechnology, in particular, the application relates to Trichoderma chlamydospore microcapsule preparation and a preparation method thereof. Background technique [0002] There are many kinds of cruciferous crops, which are closely related to people's life and have extensive economic value. Clubroot is a worldwide soil-borne disease caused by Plasmodium brassicae, which can infect a variety of cruciferous crops and cause significant losses to agricultural production. Clubroot is a serious hazard to cruciferous crops. Dormant spores formed by pathogenic bacteria can exist in the soil for a long time, making it difficult to control. Several chemical and biological pesticides that can prevent and control clubroot have been developed, combined with traditional treatment methods , have certain effects on the prevention and treatment of clubroot, but there is still no great progress in completely eradica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00
Inventor 王伟马新于学新赵永田裴文亮
Owner EAST CHINA UNIV OF SCI & TECH
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