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Primers and methods for identifying mammalian meat

A mammal and meat technology, applied in the field of primers for identifying mammal meat, can solve the problems of harming the legitimate interests of consumers, and achieve the effects of efficient identification, less restrictions, and a wide range of applications

Active Publication Date: 2019-10-18
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Driven by huge profits, unscrupulous companies use relatively low-priced meat to pass off high-priced varieties for sale to make huge profits. This practice of shoddy and shoddy has seriously damaged the legitimate interests of consumers.

Method used

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  • Primers and methods for identifying mammalian meat
  • Primers and methods for identifying mammalian meat
  • Primers and methods for identifying mammalian meat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1. Special primers and methods for mammalian meat identification

[0032] 1. Special primers for mammalian meat identification

[0033] 1. Identification of special primers

[0034] Design universal primers for mammalian COI genes based on COI genes, combined with sequencing and sequence comparison technologies, can realize the classification and identification of mammals at the nucleic acid level.

[0035] Mammalian COI gene universal primers consist of an upstream primer set and a downstream primer set,

[0036] The upstream primer set consists of upstream primer 1, upstream primer 2, upstream primer 3 and upstream primer 4:

[0037] Upstream primer 1: TGTAAAACGACGGCCAGTATTCAACCAATCATAAAGATATTGG (SEQ ID NO: 1);

[0038] Upstream primer 2: TGTAAAACGACGGCCAGTTTCTCAACCAACCACAAAGACATTGG (SEQ ID NO: 2);

[0039] Upstream primer 3: TGTAAAACGACGGCCAGTTTCTCAACCAACCACAARGAYATYGG (SEQ ID NO: 3);

[0040] Upstream primer 4: TGTAAAACGACGGCCAGTTTCTCAACCAACCAIAAIGAI...

Embodiment 2

[0069] Embodiment 2, mammalian meat nucleic acid identification

[0070] Experimental materials: Collect commercially available mammalian meat samples: pork, beef, mutton and rabbit.

[0071] 1. Genomic DNA extraction

[0072] The genomic DNA of pork, beef, mutton and rabbit meat was extracted respectively according to the method of 2 of 1 in Example 1.

[0073] 2. COI gene PCR amplification

[0074] The genomic DNAs of pork, beef, mutton and rabbit meat were respectively used as templates, and PCR amplification was carried out by the method in Example 1, to obtain PCR amplification products of different samples.

[0075] 3. Sequencing and comparison of amplified products

[0076] The PCR amplification products of different samples obtained in the above 2 were detected by gel electrophoresis with 1.5% agarose, and the results were as follows: figure 1 as shown, figure 1 The representation of each lane is shown in Table 1, and ntc is a negative control. It can be seen that...

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Abstract

The invention discloses primers and a method for identifying mammal meat. The primers for identifying the mammal meat comprise an upstream primer 1, an upstream primer 2, an upstream primer 3, an upstream primer 4, a downstream primer 1, a downstream primer 2, a downstream primer 3 and a downstream primer 4. The primers, a kit and the method provided by the invention can be used for accurately identifying the animal sources of various mammal meat within a short time, therefore, a novel thought and a novel direction are provided for the field of food detection, meanwhile, a simple, rapid and accurate approach is provided for efficiently identifying the daily meat, and moreover, a novel method and a novel means are provided for the supervision and administration for the meat market.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to primers and methods for identifying mammalian meat. Background technique [0002] In recent years, with the rapid development of society, people's living standards have also been greatly improved, and the demand for agricultural and sideline products including meat has increased significantly. At the same time, the pace of development of the food industry has been further accelerated. Driven by huge profits, unscrupulous companies use relatively low-priced meat to pass off high-priced varieties for sale to make huge profits. This practice of shoddy and shoddy meat seriously damages the legitimate interests of consumers. Therefore, it is particularly important to accurately identify meat as shoddy and shoddy. [0003] At present, the methods for mammalian meat identification mainly include: sensory identification method, spectral identification method, chromatography iden...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 朱宝利律娜秦宇轩胡永飞王黎明李晶张瑞芬
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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