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A kind of preparation method of total flavonoids of ramie

A technology of twigs and total flavonoids is applied in the field of separation and purification of plants, which can solve problems such as waste of resources, and achieve the effects of good product quality, high separation efficiency and high extraction efficiency.

Active Publication Date: 2020-06-09
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no better use of these plants, resulting in a great waste of resources

Method used

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  • A kind of preparation method of total flavonoids of ramie
  • A kind of preparation method of total flavonoids of ramie
  • A kind of preparation method of total flavonoids of ramie

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preparation example Construction

[0016] The present embodiment provides a preparation method of total flavonoids of ramie, comprising the following steps:

[0017] Pretreatment step: select the whole herb or the whole herb with roots harvested from July to September, wash, dry, and pulverize to obtain the powder of the sage.

[0018] The inventor has studied the content of total flavonoids in the seeds of the sage branch in different periods, and found that the flavonoid content in the whole herb or the whole plant of the sage branch harvested from July to September is relatively high, therefore, in the pretreatment step, July is selected -The whole herb or whole herb with roots harvested in September can increase the yield of the total flavonoids of the sage. In a preferred embodiment of the present invention, after pulverization, the particle size of the branched edulis powder is 40 mesh-60 mesh, which is beneficial to improve the extraction efficiency.

[0019] Rhizoma ramie includes ramie ramie, ramrodil...

Embodiment 1

[0031]Step 1: Select the whole herb of Cedarwort with roots harvested in July, wash, dry, and crush it to 40 mesh to obtain the powder of Crazy clover.

[0032] Step 2: Put 5 g of the ramie powder obtained in step 1 into a 24 mL extraction kettle, and extract by supercritical fluid extraction. Among them, the extraction agent is supercritical fluid CO 2 , the entraining agent is absolute ethanol, the extraction pressure is 30MPa, the extraction temperature is 40°C, the extraction time is 60min, and the volume of entraining agent added per gram of Fructus Peaches powder is 1mL. After extraction, the extract of the branch of the branch of the branch of the branch of the branch of the branch is obtained, and the absorbance at the wavelength of 510nm is measured by the ultraviolet spectrophotometer method, and the extraction amount of the flavonoids in the branch of the branch of the branch of the branch of the branch of the branch of the branch is calculated.

[0033] Step 3: af...

Embodiment 2

[0035] Step 1: Select 8 parts of the harvested whole herb of Herba Cinnamomum acuminatum with roots, wash and dry it, and crush it to 50 meshes to obtain a powder of Escarina acuminatum.

[0036] Step 2: Put 5 g of the ramie powder obtained in step 1 into a 24 mL extraction kettle, and extract by supercritical fluid extraction. Among them, the extraction agent is supercritical fluid CO 2 , the entraining agent is absolute ethanol, the extraction pressure is 40MPa, the extraction temperature is 45°C, the extraction time is 80min, and the volume of entraining agent added per gram of Fructus Peaches powder is 2mL. After extraction, the extract of the branch of the branch of the branch of the branch of the branch of the branch is obtained, and the absorbance at the wavelength of 510nm is measured by the ultraviolet spectrophotometer method, and the extraction amount of the flavonoids in the branch of the branch of the branch of the branch of the branch of the branch of the branch ...

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Abstract

The invention provides a preparation method of lespedeza total flavone. The preparation method comprises the following steps: an extraction step: taking lespedeza powder, and extracting the lespedeza powder by adopting a supercritical fluid extraction method by taking supercritical fluid CO2 as an extracting agent and an organic solvent as an entrainer to obtain a lespedeza extract; and a purification step: purifying the lespedeza extract by adopting an adsorption chromatography, performing elution by using an organic solvent mixed solution as an eluting agent to obtain an eluant, and then removing the eluting agent from the eluant to obtain lespedeza total flavone. According to the method, the supercritical fluid extraction method is adopted to extract the lespedeza powder, so that double effects of extraction and separation can be achieved, and the extraction efficiency is high; and the adsorption chromatography is adopted to separate the obtained extract, so that the impurities in the extract can be effectively removed, flavone active substances in lespedeza can be kept, the separation efficiency is high, and the obtained lespedeza total flavone is high in purity.

Description

technical field [0001] The invention relates to the field of separation and purification of plants, in particular to a preparation method of total flavonoids from ramie. Background technique [0002] Our country is rich in forage grass and grassland plant resources. In recent years, in order to prevent soil erosion, some shrubs and semi-shrubs with good resistance have become the first choice for soil and water conservation. At present, there is no better utilization of these plants, resulting in a great waste of resources. Therefore, excavating natural compounds with significant activity from these plants and developing and utilizing them will not only greatly increase their economic added value, but also have important ecological and social significance. [0003] Lespedaza hedysaroides (Pall.) Kitag.) is a small semi-shrub in the form of medium xerophytic herb. It often becomes the dominant species or associated species in meadow steppe communities in forest and forest st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P1/16A61P39/06A61P43/00A61P31/12
CPCA61K36/48A61K2236/37
Inventor 吴洪新阿拉木斯常春戴雅婷张小庆赵金梅乌兰巴特儿孙娟娟刘洪林
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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