Novel lamellar molybdenum disulfide-based nano-immune adjuvant and its preparation method and application

A molybdenum disulfide, immune adjuvant technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, antibody medical ingredients, etc. Toxicity, enhanced immune response, increased secretion levels

Active Publication Date: 2019-11-08
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its own series of shortcomings, the current research level of aluminum immune adjuvants can no longer meet the needs of the rapid development of vaccines. Therefore, the development of new immune immune adjuvants is particularly important

Method used

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  • Novel lamellar molybdenum disulfide-based nano-immune adjuvant and its preparation method and application
  • Novel lamellar molybdenum disulfide-based nano-immune adjuvant and its preparation method and application
  • Novel lamellar molybdenum disulfide-based nano-immune adjuvant and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A. Preparation of molybdenum disulfide nanosheets

[0065] (1) Weigh 500mg of molybdenum disulfide powder (99%) and place it in a 10mL dry round-bottomed flask, feed nitrogen, add 5mL of 1.6mol / L n-butyllithium dispersed in hexane, and react at room temperature for 48h;

[0066] (2) After the reaction is over, add hexane, dilute the reaction solution, and centrifuge for 10 min; disperse the lower layer of sediment with hexane, and centrifuge twice;

[0067] (3) Take the lower layer of precipitate and disperse it in aqueous solution, sonicate until no bubbles are generated; centrifuge for 10 minutes, remove the lower layer, take the supernatant, and dialyze with a dialysis membrane with a molecular cut-off of 14kDa for 48 hours to obtain molybdenum disulfide nanosheets with less than 10 sheets aqueous solution.

[0068] B. Preparation of molybdenum disulfide-based nano-immune adjuvant with small particle size and uniform size

[0069] (1) Ultrasonically operate the mol...

Embodiment 2

[0084] A. Preparation of molybdenum disulfide nanosheets

[0085] (1) Weigh 500mg of molybdenum disulfide powder (99%) and place it in a 10mL dry round-bottomed flask, feed nitrogen, add 5mL of 1.6mol / L n-butyllithium dispersed in hexane, and react at room temperature for 48h;

[0086] (2) After the reaction is over, add hexane, dilute the reaction solution, and centrifuge for 10 min; disperse the lower layer of sediment with hexane, and centrifuge twice;

[0087] (3) Take the lower layer of precipitate and disperse it in aqueous solution, sonicate until no bubbles are generated; centrifuge for 10 minutes, remove the lower layer, take the supernatant, and dialyze with a dialysis membrane with a molecular cut-off of 14kDa for 48 hours to obtain molybdenum disulfide nanosheets with less than 10 sheets aqueous solution.

[0088] B. Preparation of molybdenum disulfide-based nano-immune adjuvant with small particle size and uniform size

[0089] (1) Ultrasonically operate the mol...

Embodiment 3

[0104] A. Preparation of molybdenum disulfide nanosheets

[0105] (1) Weigh 500mg of molybdenum disulfide powder (99%) and place it in a 10mL dry round-bottomed flask, feed nitrogen, add 5mL of 1.6mol / L n-butyllithium dispersed in hexane, and react at room temperature for 48h;

[0106] (2) After the reaction is over, add hexane, dilute the reaction solution, and centrifuge for 10 min; disperse the lower layer of sediment with hexane, and centrifuge twice;

[0107] (3) Take the lower layer of precipitate and disperse it in aqueous solution, sonicate until no bubbles are generated; centrifuge for 10 minutes, remove the lower layer, take the supernatant, and dialyze with a dialysis membrane with a molecular cut-off of 14kDa for 48 hours to obtain molybdenum disulfide nanosheets with less than 10 sheets aqueous solution.

[0108] B. Preparation of molybdenum disulfide-based nano-immune adjuvant with small particle size and uniform size

[0109] (1) Ultrasonically operate the mol...

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Abstract

The invention relates to a novel lamellar molybdenum disulfide based nano immunologic adjuvant as well as a preparation method and application thereof. The immunologic adjuvant is a product obtained by modifying a molybdenum disulfide nano lamina by using CpG and PEG. The preparation method comprises: performing ultrasonic treatment on the molybdenum disulfide nano lamina; then mixing the molybdenum disulfide nano lamina with CpG and PEG, and performing temperature controlled incubation in a swing bed; and performing centrifugation to remove free CpG and PEG. The nano immunologic adjuvant provided by the invention is small in particle size, uniform in size, and large in specific area, has relatively strong near-infrared region photo-thermal conversion capability, and can improve the secretion level of immune activation related cell factors to a great extent in combination with infrared laser irradiation. Moreover, the nano immunologic adjuvant provided by the invention has relatively low cytotoxicity. The nano immunologic adjuvant provided by the invention has the advantages of low raw material cost, simple preparation process, good repeatability, stable performance and the like.

Description

technical field [0001] The invention belongs to the field of preparation of novel nano-immune adjuvants, and relates to a sheet-layer molybdenum disulfide-based nano-immune adjuvant and its preparation method and application, in particular to a CpG-modified sheet-layer molybdenum disulfide-based nano-adjuvant with high photothermal conversion efficiency. Molybdenum sulfide nano-immune adjuvant and its preparation method and application. Background technique [0002] As a new treatment strategy, immunotherapy has developed into the fourth tumor treatment method after surgery, radiotherapy and chemotherapy. Because it is based on the monitoring and clearance function of the body's own immune system, it stimulates and enhances the body's immune function to kill Tumor cells have shown good application prospects. At the same time, due to the poor immunogenicity of most recombinant vaccines, it is difficult to induce a good immune response, and an immune adjuvant must be added to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39
CPCA61K39/39A61K2039/55505A61K2039/55511A61K2039/55561A61K2300/00
Inventor 韩秋森杨蓉王新环王琛
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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