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A kind of cultivation method of Striga
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A cultivation method, the technology of Striga, applied in the field of plant tissue cultivation, achieves the effect of large commercial application prospects and high survival rate
Active Publication Date: 2017-12-01
GUANGDONG KING ZUO AGRI SCI & TECH CO LTD
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[0004] At present, there is no report on the commercial cultivation of Striga, but Striga cultivation technology with high survival rate and fast reproduction has great commercial application prospects
Method used
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Embodiment 1
[0033] A method for cultivating Striga, comprising the following steps:
[0034] 1) Take immature capsules of Striga, put the capsules into 0.1% mercuric chloride solution for disinfection for 4 minutes, wash them three times with sterile water, each time for 2 minutes; are green capsules;
[0035] 2) On the super-workbench, crush the pericarp of the capsule of step 1) with sterile tweezers, and release the zygotic embryo;
[0036] 3) inoculating the zygotic embryos in step 2) into the induction medium, and conducting induction culture at 28±2° C. under artificial light with a light intensity of 2000 Lx;
[0037] The induction medium is MS medium containing 0.5g / L hydrolyzed casein, 0.2mg / L 6-BA and 0.05mg / L IBA, and the pH of the induction medium is 5.8±0.1.
[0038] The Striga cultivated in Example 1 developed well, figure 1 It is the growth schematic diagram of embodiment 1 induction culture after 3 weeks, from figure 1 It can be clearly seen that the zygotic embryo has...
Embodiment 2
[0040] A method for cultivating Striga, comprising the following steps:
[0041] 1) Take the mature capsule of Striga, put the capsule into 0.1% mercuric chloride solution for disinfection for 6 minutes, and wash it three times with sterile water, each time for 3 minutes; Capsules about to burst;
[0042] 2) On the super-workbench, crush the pericarp of the capsule of step 1) with sterile tweezers, and release the zygotic embryo;
[0043] 3) Inoculate the zygotic embryos in step 2) into the induction medium, and conduct induction culture at 20° C. under artificial light with a light intensity of 1500 Lx;
[0044] The induction medium is MS medium containing 0.8g / L hydrolyzed casein, 0.1mg / L 6-BA and 0.06mg / L IBA, and the pH of the induction medium is 5.5-6.0.
[0045] The Striga in this example grows a complete plant after 11 weeks of induced culture.
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Abstract
The invention provides an asiatic witchweed cultivation method. The asiatic witchweed cultivation method includes the following steps that 1, asiatic witchweed capsules are taken, disinfected and cleaned with sterile water; 2, zygote embryos are taken from the capsules in the step 1; 3, the zygote embryos in the step 2 are inoculated to an inducing culture medium for inducing culture. The inducing culture medium is an MS culture medium containing casein hydrolysate, 0.1-0.3 mg / L of 6-BA and 0.03-0.06 mg / L IBA, and the pH of the inducing culture medium is 5.5-6.5. The asiatic witchweed cultivation method utilizes the low-concentration 6-BA and IBA to induce germination of the zygote embryos of asiatic witchweed, aboveground parts are firstly formed, and then roots of the basic parts grow out slowly to form complete plants. The asiatic witchweed cultivation method can rapidly reproduce and cultivate the asiatic witchweed at high survival rate and has a great commercial application prospect.
Description
technical field [0001] The invention relates to the field of plant tissue cultivation, in particular to a method for cultivating striga. Background technique [0002] Strigaasiatica (L.) O.Kuntze, also known as Strigaasiatica (L.) O.Kuntze, is also known as Strigaasiatica (L.) O.Kuntze, etc. Scrophulariaceae Striga metal plant, annual semi-parasitic herb, mostly distributed in low-altitude barren mountains, grasslands, Tianbian and other places, often parasitic on the roots of grasses and other herbaceous plants. Striga has the effect of clearing heat and eliminating accumulation, and is a traditional folk medicinal plant. It is used as a commonly used pediatric medicine in Guangdong and Guangxi of my country, and is widely used in the treatment of infantile malnutrition, excessive liver fire, hepatitis, infantile diarrhea, night blindness, anorexia, anti-inflammation, etc. The main medicinal ingredients include flavonoids and other compounds, polysaccharides, etc. , its po...
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