Preparation method of targeted microbubble ultrasound contrast agent modified by single-chain antibody

Abstract

The invention belongs to the field of medical science, and relates to a single chain antibody-modified targeting micro-bubble ultrasonic contrast agent and a preparation method thereof. The preparation method comprises the following steps: preparing a maleimidized liposome; opening the disulfide bond of an antibody by using a reducing agent to form a mercapto group-exposed single chain antibody; linking the single chain antibody to the surface of the liposome to form a targeting liposome; and preparing the targeting micro-bubble ultrasonic contrast agent through a mechanical oscillation technology. The method can effectively improve the yield of the targeting micro-bubble ultrasonic contrast agent, and has an important application prospect in the invention and in relevant targeting micro-bubble ultrasonic contrast agent industrial production processes.

Description

technical field

[0001] The invention relates to the fields of ultrasonic molecular imaging and biomedical engineering, in particular to a method for preparing a lipid microbubble ultrasonic contrast agent modified by a single-chain antibody.

[0002] technical background

[0003] Ultrasound molecular imaging is a molecular imaging method that uses targeted microbubble contrast agents as molecular probes and uses contrast-enhanced ultrasound as an imaging mode to conduct qualitative and quantitative research on the biochemical processes in vivo at the cellular and molecular levels. Accuracy and sensitivity have important application prospects in the fields of cardiovascular, inflammatory, tumor and other diseases. Among them, targeted ultrasonic molecular probes are the key to ultrasonic molecular imaging technology. Ultrasound molecular probes reported in the literature are mainly microbubble ultrasound contrast agents: specific ligands (antibodies, polypeptides, nucleic aci...

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