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Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof

A technology of cellulosic ethanol and composite bacterial agents, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of low final yield of ethanol, high production cost of cellulosic ethanol fermentation, loss of wood The nutrient content of cellulose and other issues can be achieved to reduce production costs, increase the final yield, and reduce production costs

Active Publication Date: 2010-03-10
江苏哈宜环保研究院有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high production cost of cellulose ethanol fermentation caused by washing and detoxification method and the loss of nutrients in lignocellulose by washing and detoxification method resulting in ethanol In order to solve the problem of low final yield, a kind of microbial composite bacteria agent and preparation method thereof for cellulosic ethanol production are provided

Method used

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  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof
  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof
  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof

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specific Embodiment approach 1

[0011] Specific embodiment one: the present embodiment is used for the microbial compound bacteria agent of cellulosic ethanol production mainly by the concentration being 10 8 Individual / mL Trichoderma korningii spore suspension, the concentration is 10 8 Aspergillus oryzae spore suspension and concentration of 10 per mL 8 Chaetomium chaetomium spore suspension per mL was prepared according to the volume ratio of 1:1:1.

[0012] The preparation method of the Trichoderma korningii spore suspension in the present embodiment is: Trichoderma korningina is inoculated on the slant of potato dextrose agar medium, cultivates 48~72h under the condition of 28~32 DEG C to obtain the spores of Trichoderma korningina, and then use Bacteria water washes the Trichoderma korningina spores cultivated on the slant of potato dextrose agar medium, and evenly mixes sterile water with the Trichoderma korningina spores to obtain the Trichoderma korningina spore suspension, wherein the Trichoderma ...

specific Embodiment approach 2

[0018] Specific embodiment two: the difference between this embodiment and specific embodiment one is that the microbial compound bacterial agent used for cellulosic ethanol production also includes a culture medium, and the culture medium consists of 7 to 9 parts by weight of bran, 11 to 13 parts by weight. It consists of 14-16 parts of microcrystalline cellulose and 14-16 parts of inorganic salt solution. Others are the same as the first embodiment.

[0019] The preparation method of the culture medium in this embodiment is as follows: mix 7 to 9 parts of bran, 11 to 13 parts of microcrystalline cellulose and 14 to 16 parts of inorganic salt solution according to the ratio of parts by weight, and then place the mixture at 121 Under the condition of ℃, sterilize for 20 minutes to obtain the culture medium.

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment two is that KH in the inorganic salt solution 2 PO 4 The mass concentration is 0.7%, (NH 4 ) 2 SO 4 The mass concentration is 3.3%, K 2 HPO 4 The mass concentration is 0.7% and MgSO 4 The mass concentration is 0.1% aqueous solution, and the pH value of the inorganic salt solution is 5-7. Others are the same as the first embodiment.

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Abstract

The invention discloses a microbial compound inoculum for the production of cellulosic ethanol and a preparation method thereof, relating to a compound inoculum and a preparation method thereof, and solving the problems that the cost of fermentation production of cellulosic ethanol is high due to the adoption of a rinsing and detoxification method, and the ultimate yield of the ethanol is low because the rinsing and detoxification method loses the nutrition elements in the lignocellulose. The microbial compound inoculum is mainly prepared from a trichoderma koningii spore suspension, an aspergillus oryzae spore suspension and a chaetomium cupreum spore suspension. The preparation method comprises the following steps: firstly, mixing the trichoderma koningii spore suspension, the aspergillus oryzae spore suspension and the chaetomium cupreum spore suspension; and secondly, mixing and inoculating the mixed spore suspension in a culture medium for cultivation to obtain the microbial compound inoculum. The microbial compound bacterium has few dosage and reduces the cost of the fermentation production of cellulosic ethanol, and the ethanol prepared from the microbial compound inoculum has high ultimate yield.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent and a preparation method thereof. Background technique [0002] The annual output of crop stalks in the world exceeds 2 billion tons, and China’s annual output reaches about 700 million tons, of which the output of corn stalks is about 250 million tons. Except for a small part of these corn stalks used for animal feed and rural fuel, most of them are directly burned. It not only wastes resources, but also seriously pollutes the environment. On the other hand, with the gradual depletion of fossil energy sources, the energy crisis is intensifying, and ethanol is considered to be the best renewable liquid energy source. [0003] The existing cellulosic ethanol fermentation process uses cellulose in crop straws for fermentation. This fermentation process uses renewable cellulose resources as raw materials for ethanol production research, which not only alleviates the energy crisis, but also red...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/22C12P39/00C12P7/10C12R1/885C12R1/69C12R1/645
CPCY02E50/16Y02E50/10
Inventor 冯玉杰于艳玲刘嘉李冬梅徐琛
Owner 江苏哈宜环保研究院有限公司
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