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Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof

A technology of cellulosic ethanol and composite bacterial agents, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., to achieve the effects of reducing production costs, alleviating energy crises, and reducing water consumption

Inactive Publication Date: 2011-06-15
江苏哈宜环保研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of high production cost of cellulose ethanol fermentation caused by washing and detoxification method and the loss of nutrients in lignocellulose by washing and detoxification method resulting in ethanol In order to solve the problem of low final yield, a kind of microbial composite bacteria agent and preparation method thereof for cellulosic ethanol production are provided

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  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof
  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof
  • Microbial compound inoculum for production of cellulosic ethanol and preparation method thereof

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specific Embodiment approach 1

[0015] The microbial composite bacterial agent of the present embodiment reacts with formic acid, acetic acid, malic acid, hydroxymethylfurfural (HMF) and phenolic substances respectively, and the result after the reaction shows that the composite bacterial agent of the present invention formic acid, acetic acid, malic acid, hydroxyl The degradation rates of methylfurfural (HMF) and phenols were 72.37% to 81.69%, 50.61% to 60.89%, 78.18% to 89.12%, 98% to 100% and 70.08% to 86.23%. From these data, it can be seen that It can be seen that the composite bacterial agent of the present invention is used in the production process of cellulose ethanol, and has a good removal effect on formic acid, acetic acid, malic acid and phenolic compounds produced in the pretreatment process of corn stalks. The detoxification effect is good.

[0016] The steps of utilizing the microbial composite bacterial agent of the present embodiment to carry out the cellulosic ethanol production process ar...

specific Embodiment approach 2

[0023] Specific embodiment five: what this embodiment is different from specific embodiment four is that the preparation method of Trichoderma korningii spore suspension in step 1 is: Trichoderma korningii is inoculated on the slant of potato dextrose agar medium, at 28~32 ℃ of conditions Cultivate for 48-72 hours to obtain Trichoderma korningina spores, then wash the Trichoderma korningina spores obtained by culturing on the slant of potato dextrose agar medium with sterile water, and mix the sterile water and Trichoderma korningina spores evenly to obtain Trichoderma korningina spores Mold spore suspension. Other steps and parameters are the same as those in Embodiment 4.

[0024] Specific embodiment six: what this embodiment is different from specific embodiment four or five is that the preparation method of aspergillus oryzae spore suspension in step one is: aspergillus oryzae is inoculated on the slant of potato dextrose agar medium, at 28~32 ℃ of conditions Cultivate fo...

specific Embodiment approach 3

[0025] Specific embodiment seven: the difference between this embodiment and specific embodiment six is ​​that the preparation method of Chaetomium spore suspension in step 1 is: inoculate Chaetomium on the slant of potato dextrose agar medium, in 28~32 Cultivate at ℃ for 48-72 hours to obtain Chaetomium spores, then wash the Chaetomium spores obtained by culturing on the potato dextrose agar medium slope with sterile water, and mix the sterile water and Aspergillus oryzae spores evenly. A suspension of Chaetomium spores was obtained. Other steps and parameters are the same as those in Embodiment 6.

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Abstract

The invention discloses a microbial compound inoculum for the production of cellulosic ethanol and a preparation method thereof, relating to a compound inoculum and a preparation method thereof, and solving the problems that the cost of fermentation production of cellulosic ethanol is high due to the adoption of a rinsing and detoxification method, and the ultimate yield of the ethanol is low because the rinsing and detoxification method loses the nutrition elements in the lignocellulose. The microbial compound inoculum is mainly prepared from a trichoderma koningii spore suspension, an aspergillus oryzae spore suspension and a chaetomium cupreum spore suspension. The preparation method comprises the following steps: firstly, mixing the trichoderma koningii spore suspension, the aspergillus oryzae spore suspension and the chaetomium cupreum spore suspension; and secondly, mixing and inoculating the mixed spore suspension in a culture medium for cultivation to obtain the microbial compound inoculum. The microbial compound bacterium has few dosage and reduces the cost of the fermentation production of cellulosic ethanol, and the ethanol prepared from the microbial compound inoculum has high ultimate yield.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent and a preparation method thereof. Background technique [0002] The annual output of crop stalks in the world exceeds 2 billion tons, and China’s annual output reaches about 700 million tons, of which the output of corn stalks is about 250 million tons. Except for a small part of these corn stalks used for animal feed and rural fuel, most of them are directly burned. It not only wastes resources, but also seriously pollutes the environment. On the other hand, with the gradual depletion of fossil energy sources, the energy crisis is intensifying, and ethanol is considered to be the best renewable liquid energy source. [0003] The existing cellulosic ethanol fermentation process uses cellulose in crop straws for fermentation. This fermentation process uses renewable cellulose resources as raw materials for ethanol production research, which not only alleviates the energy crisis, but also re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/22C12P39/00C12P7/10C12R1/645C12R1/69C12R1/885
CPCY02E50/16Y02E50/10
Inventor 冯玉杰于艳玲刘嘉李冬梅徐琛
Owner 江苏哈宜环保研究院有限公司
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