Oolong tea polyphenol and extraction method thereof

An extraction method and technology of tea polyphenols, applied in the field of oolong tea polyphenols and its extraction, to achieve the effects of high extraction efficiency, stable properties, and efficient reaction

Active Publication Date: 2016-08-17
茶弈会(天津)技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology involves carrying out chemical reactions on green tea leaf with different types of cells called chitinases (cell walls) or carbohemoglobin proteins. These processes help break down certain compounds from them into smaller molecules like sugars while maintaining their original structure. By controlling these steps during processing, the resulting product contains various flavonoids such as quercetone gamma glucosides, lignans, tannan polysaccharides, etc., along with other substances found naturally within teas.

Problems solved by technology

The technical problem addressed in this patented patent relates to improving the efficient production and separation of teapolyphenols (TPPs), particularly those containing carboxylic acids called gallins that cause damage or degraded flavors due to heat exposures. Existing methods involve complicated processes like centrifugal processing, filtering out unwanted substances beforehand, and various techniques involving different materials including organosilicon compounds, metal ion chelators, aminoaccharides, protease stabilizers, and sugar esters. These issues make conventional methods less effective than desired because these components may interfere negatively towards each other' effectiveness upon further use.

Method used

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  • Oolong tea polyphenol and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A kind of extraction method of oolong tea tea polyphenol

[0037] Include the following steps:

[0038] Step S1, disinfection: soak and wash the oolong tea leaves in clear water for 2 hours, remove them and dry them in the sun, and use 1% acetic acid mist to fumigate the oolong tea leaves for 20 minutes;

[0039] Step S2, sterilization: use a 300W ultraviolet lamp to sterilize the sterilized oolong tea leaves for 5 minutes;

[0040] Step S3, enzymatic hydrolysis: put the sterilized oolong tea leaves into an aqueous cellulase solution with a mass fraction of 5%, adjust the pH value of the solution to 4.5 using a sodium dihydrogen phosphate / citric acid buffer solution with a pH of 3.5, and perform enzymatic hydrolysis 3h, after the enzymatic hydrolysis is completed, raise the temperature to 96°C for 25min to inactivate the enzyme, then collect the oolong tea enzymatic hydrolyzate for later use;

[0041] Step S4, preparation of tea polyphenols crude extract: use 0.1% dil...

Embodiment 2

[0045] A kind of extraction method of oolong tea tea polyphenol

[0046] Include the following steps:

[0047] Step S1, disinfection: soak and wash the oolong tea leaves in clear water for 3 hours, remove them and dry them in the sun, and use 10% acetic acid mist to fumigate the oolong tea leaves for 60 minutes;

[0048] Step S2, sterilization: use a 500W ultraviolet lamp to sterilize the sterilized oolong tea leaves for 10 minutes;

[0049] Step S3, enzymatic hydrolysis: put the sterilized oolong tea leaves into a cellulase aqueous solution with a mass fraction of 20%, adjust the pH value of the solution to 6.2 with a sodium dihydrogen phosphate / citric acid buffer solution with a pH of 3.7, and perform enzymatic hydrolysis 8h, after the enzymatic hydrolysis is completed, raise the temperature to 96°C for 25min to inactivate the enzyme, and then collect the oolong tea enzymatic hydrolyzate for later use;

[0050] Step S4, preparation of tea polyphenols crude extract: use 0.5...

Embodiment 3

[0054] A kind of extraction method of oolong tea tea polyphenol

[0055] Include the following steps:

[0056] Step S1, disinfection: soak and wash the oolong tea leaves in clear water for 2.5 hours, remove them and dry them in the sun, and use 5.5% acetic acid mist to fumigate the oolong tea leaves for 40 minutes;

[0057] Step S2, sterilization: use a 400W ultraviolet lamp to sterilize the sterilized oolong tea leaves for 7.5 minutes;

[0058] Step S3, enzymatic hydrolysis: put the sterilized oolong tea leaves into a cellulase aqueous solution with a mass fraction of 12.5%, adjust the pH value of the solution to 5.4 with a sodium dihydrogen phosphate / citric acid buffer solution with a pH of 3.6, and perform enzymatic hydrolysis After 5.5 hours, after the enzymatic hydrolysis is completed, heat up to 96°C for 25 minutes to inactivate the enzyme, and then collect the oolong tea enzymatic hydrolysis solution for later use;

[0059] Step S4, preparation of tea polyphenols crud...

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PUM

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Abstract

The invention discloses an Oolong tea polyphenol and an extraction method thereof. The extraction method comprises the following steps: carrying out fumigation sterilization on Oolong tea leaves by using acetic acid mist; carrying out germicidal treatment by using an ultraviolet lamp; adding into a cellulase water solution, regulating the pH value of the solution by using a sodium dihydrogen phosphate/citric acid buffer solution, carrying out enzymolysis, and heating to inactivate the enzyme; regulating the pH value of the enzymolysis solution by using dilute hydrochloric acid, heating, cooling, passing through a semi-permeable membrane to filter out insoluble impurities, and carrying out separation purification on the filtrate by carbon dioxide supercritical fluid chromatography to obtain a tea polyphenol crude extracting solution; carrying out hydrodistillation removal treatment on the tea polyphenol crude extracting solution to remove caffeine impurities, thereby obtaining the purified tea polyphenol refined solution; and carrying out reduced pressure distillation on the tea polyphenol refined solution to remove moisture, thereby obtaining the Oolong tea polyphenol. The method can extract and purify the tea polyphenol in the Oolong tea fannings, thereby enhancing the utilization ratio of the Oolong tea fannings and increasing the product value and industrial value of the Oolong tea.

Description

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Claims

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Application Information

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Owner 茶弈会(天津)技术有限公司
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