Method for permanently embedding identity card number into genome

Abstract

The invention provides a method for permanently embedding an identity card number into a genome. The method comprises the following steps: permanently embedding an exogenous gene segment containing an identity card number into a genome by using CRISPR technology; and separating and reading specific DNA containing the identity card number. Thus, identity can always be identified via the permanently preserved DNA even an external packaging label is lost or wrong. The method is also applicable to preservation of microbes (including bacteria and viruses). For example, if a deactivated SARS virogene specimen is left, later generations can research on SARS viruses.

Description

technical field [0001] The invention belongs to the field of gene extraction, transportation and preservation, and in particular relates to a method for permanently embedding an ID card number into a genome. Background technique [0002] The current gene extraction, transportation and storage mainly rely on the labels on the test tubes. Due to the many links, the labels written on the outside of the test tubes are easy to be mistaken or worn out. Ensuring that the identity of a gene can never be mistaken is crucial to the establishment of a national gene bank. Therefore, we have to permanently paste the ID number edited with DNA on the gene. In this way, even if the test tube does not have any label, as long as the sample is there, the ID number is there. [0003] There are currently two ways to convert data information into DNA information for permanent storage. One is not to insert the gene sequence directly into the genome, but to mix the chemical substance with the ge...

AI Technical Summary

Problems solved by technology

One is not to insert the gene sequence directly into the genome, but to mix the chemical substance with the genome sample, and use the structure of the chemical substance to represent the data information. The disadvantage of this method is that when analyzing the data information, not only the detection of the chemical substance , but also to test the genome sample, which is more troublesome

[0004] The other is not to use the CRISPR-Cas9 method, but directly use the restriction enzyme cutting method to cut the genome and then connect the sequence representing the ID number to the genome. The disadvantage of this method is that the base of the restriction enzyme is only 4- 6, so the accuracy of targeting cannot be guaranteed

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