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A chromogenic medium for detecting Enterobacter sakazakii

A chromogenic medium, the technology of Enterobacter sakazakii, is applied in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc. , to achieve the effect of short detection period, strong specificity and high sensitivity

Active Publication Date: 2020-04-07
WUXI SAIWEI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On DFI agar (Enterobacter sakazakii chromogenic medium), the α-glucosidase produced by Enterobacter sakazakii reacts with the chromogenic substrate in the medium to form a colorless colony with a green center, but due to Proteus vulgaris It can also form colonies with green centers on DFI agar, causing interference; the appearance of DFI agar plates is brownish yellow, with small color differences and difficult to observe
A domestic brand of Enterobacter sakazakii chromogenic medium will appear point crystals after being placed at low temperature, which is suspected of bacterial contamination and affects the appearance
Chromogenic medium for Enterobacter sakazakii from CHROMagar, France also affects its specificity because Proteus mirabilis can produce spreading green colonies

Method used

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  • A chromogenic medium for detecting Enterobacter sakazakii
  • A chromogenic medium for detecting Enterobacter sakazakii
  • A chromogenic medium for detecting Enterobacter sakazakii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A chromogenic medium for detecting Enterobacter sakazakii, each 1000ml medium contains 10g of tryptone, 5g of beef extract powder, 5g of NaCl, 16g of bacterial agar powder, 0.4g of sodium deoxycholate, and 0.4g of sodium thiosulfate , 0.13 g of 5-bromo-4-chloro-3-indole-α-D-glucoside, and 3 ml of N,N-dimethylformamide.

[0028] According to Example 1, a total of 7.35g of this product was weighed and dissolved in 200ml of water, boiled and cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0029] According to Example 1, a total of 7.35 g of this product was weighed and dissolved in 200 ml of water, autoclaved at 121°C for 15 minutes, cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0030] The standard strains of Enterobacter sakazakii ATCC29544 and ATCC51392 were streaked and inoculated on the color plates of Enterobacter sakazakii prepared by two sterili...

Embodiment 2

[0063] A chromogenic medium for detecting Enterobacter sakazakii, each 1000ml medium contains tryptone 15g, beef extract powder 7g, NaCl 5g, bacterial agar powder 18g, sodium deoxycholate 0.6g, sodium thiosulfate 1.0g , 0.1 g of 5-bromo-4-chloro-3-indole-α-D-glucoside, and 1 ml of N,N-dimethylformamide.

[0064] According to Example 2, a total of 7.35g of this product was weighed and dissolved in 200ml of water, boiled and cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0065] According to Example 2, a total of 7.35g of this product was weighed and dissolved in 200ml of water, autoclaved at 121°C for 15 minutes, cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0066] The standard strains of Enterobacter sakazakii ATCC29544 and ATCC51392 were streaked and inoculated on the color plates of Enterobacter sakazakii prepared by two sterilization methods. Autoclav...

Embodiment 3

[0083] A chromogenic medium for detecting Enterobacter sakazakii, each 1000ml medium contains tryptone 20g, beef extract powder 10g, NaCl 5g, bacterial agar powder 20g, sodium deoxycholate 0.2g, sodium thiosulfate 0.2g , 0.08 g of 5-bromo-4-chloro-3-indole-α-D-glucoside, and 10 ml of N,N-dimethylformamide.

[0084] According to Example 3, a total of 7.35g of this product was weighed and dissolved in 200ml of water, boiled and cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0085] According to Example 3, a total of 7.35 g of this product was weighed and dissolved in 200 ml of water, autoclaved at 121°C for 15 minutes, cooled to about 50°C, poured into a sterile plate, and the prepared plate was stored at 2-8°C in the dark.

[0086] The standard strains of Enterobacter sakazakii ATCC29544 and ATCC51392 were streaked and inoculated on the color plates of Enterobacter sakazakii prepared by two sterilization methods. Aut...

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Abstract

The invention discloses a chromogenic culture medium for detecting Enterobacter sakazakii. The culture medium comprises 10-25 g / L peptone, 2-10 g / L beef extract powder, 3-10 g / L NaCl, 15-20 g / L bacteria agar powder, 0.2-1.0 g / L first inhibitor, 0.1-1.0 g / L second inhibitor, 0.1-0.3 g / L glucosaccharase chromogenic substrate and 1-10ml of cosolvent. The chromogenic culture medium for detecting Enterobacter sakazakii has the advantages of high sensitivity, high specificity, high differentiation degree, short detection period and the like, and can easily implement industrialized production.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting Enterobacter sakazakii. Background technique [0002] Enterobacter sakazakii (E. sakazakii), a gram-negative non-bacillus, is one of the normal intestinal flora of humans and animals, and is an opportunistic pathogen. It was known as Enterobacter cloacae until 1980. Until Farmer proposed that the bacterium was a new species belonging to the genus Enterobacteriaceae. In 2008, Iversen suggested that Enterobacter sakazakii should be reclassified and named as a new genus of Enterobacteriaceae. Enterobacter sakazakii can cause severe neonatal meningitis, enterocolitis and bacteremia, and the mortality rate is as high as 50%. In 1961, Franklin et al first reported two cases of meningitis caused by Enterobacter sakazakii. Since then, a series of neonatal Enterobacter sakazakii infections have been reported around the world. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/10
CPCC12Q1/045C12Q1/10Y02A50/30
Inventor 于瑞莉陈帅陈臣陈鹏飞茆伟伟
Owner WUXI SAIWEI BIOTECH CO LTD