Method for detecting carp IGF2b gene single nucleotide polymorphism and primer
A single nucleotide polymorphism and gene technology, applied in biochemical equipment and methods, microbial determination/testing, DNA/RNA fragments, etc.
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[0025] The cDNA sequence of IGF2b gene (AF402958) was retrieved from the NCBI (www.ncbi.nlm.nih.gov) database, and primers were designed (see Table 1 and Table 2).
[0026] Table 1 Primers required for amplification of carp IGF2b cDNA
[0027]
[0028]
[0029] Table 2 Primers required for amplification of carp IGF2bDNA
[0030]
[0031]The exons of the IGF2b gene cDNA sequences of 3 yellow river carp and Jian carp to be tested were amplified by the primers provided in Table 1, and then the above IGF2b gene sequences of the Yellow River carp and Jian carp were amplified by the primers provided in Table 2 The above amplified products were subjected to agarose gel electrophoresis, and then the PCR products were gel-cut, recovered, purified and sequenced, and finally spliced according to the sequence of the zebrafish genome sequence to obtain the complete IGF2b gene of the Yellow River carp and Jian carp. sequence.
[0032] The above specific process is as follows: t...
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