Fetus chromosome aneuploid genome sequencing detection method

A genome sequencing and aneuploidy technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of lack of detection methods, steps, and uneven levels of detection methods, so as to ensure accuracy, prevent Chromosomal mutation, reducing the effect of disease

Pending Publication Date: 2021-04-16
上海博奥颐和医学检验所有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a genome sequencing detection method for fetal chromosomal aneuploidy, which solves the problems of uneven levels of existing detection methods and the lack of complete detection methods and steps

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] The embodiment of the present invention provides a method for genome sequencing detection of fetal chromosomal aneuploidy, comprising the following steps:

[0028] s1: Sampling, taking peripheral blood containing fetal DNA from pregnant women;

[0029] s2: DNA extraction, DNA extraction, and concentration;

[0030] s3: Check, use high-throughput sequencing technology to scan the whole genome and conduct bioinformatics analysis;

[0031] S4: output the result.

[0032] By testing the fetus at about 12 to 22 weeks of gestation, if the fetus has chromosome deletion or polychromosomal conditions in the test, we recommend terminating the pregnancy, and the pregnancy work is relatively simple at this time, in order to ensure the safety of pregnant women and their family harmony , the present invention is through multiple rounds simultaneously, and each round carries out multiple (three times) detection, guarantees the accuracy of detection, guarantees no error, prevents tha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a fetus chromosome aneuploid genome sequencing detection method, and relates to the field of fetus chromosome detection. The fetus chromosome aneuploid-based genome sequencing detection method comprises the following steps: s1, sampling: taking peripheral blood containing fetus DNA of a pregnant woman; s2, extracting DNA: extracting DNA, and then concentrating; s3, performing inspection, scanning the whole genome by using a high-throughput sequencing technology, and performing biological information analysis; and s4, outputting a result. Abortion tissues (including embryos, fetal tissues or villi) can be collected by sampling in step s1. The fetus is detected about 12-22 weeks after pregnancy, if chromosome deletion or polychromosome occurs in the fetus detection process, it is suggested that pregnancy is terminated, the pregnancy work is simple at the moment, and the safety of pregnant women and the family harmony of the pregnant women are guaranteed.

Description

technical field [0001] The invention relates to the technical field of fetal chromosome detection, in particular to a genome sequencing detection method for fetal chromosome aneuploidy. Background technique [0002] Chromosomes are genetic material, the carrier of genes, and human autosomes exist in pairs. There are 23 pairs of somatic chromosomes in the human body, 22 of which are shared by men and women, called autosomes; the other pair is sex-determining chromosomes, which are different for men and women, called sex chromosomes, and males are XY , female is XX. In germ cells (generative cells), the composition of male germ cell chromosomes: 22 pairs of autosomes + XY. Chromosome composition of female reproductive cells: 22 pairs of autosomes + XX; aneuploidy (aneuploid) refers to the increase or decrease of one or several chromosomes in the cell. This is the most common type of chromosomal aberration in humans. A cell with one or more missing chromosomes is called hyp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12Q1/6869
Inventor万妮
Owner上海博奥颐和医学检验所有限公司