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A kind of method extracting benzyl isothiocyanate from broccoli

A technology of benzyl isothiocyanate and broccoli, applied in the field of natural product extraction, can solve the problems of complex chemical synthesis process, high equipment and cost, toxic and side effects of products, etc., achieve short extraction time, promote enzymatic reaction, The effect of increasing the extraction rate

Active Publication Date: 2020-03-20
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, benzyl isothiocyanate mainly has chemical synthesis method and natural extraction method, but the chemical synthesis method has complicated process, high equipment and cost, most of the used chemical substances are toxic, low safety factor, and the product has certain toxic and side effects
In addition, the natural extraction method in the prior art has the defects of complex process, long extraction time and low extraction rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of method of extracting benzyl isothiocyanate from broccoli of the present embodiment, it may further comprise the steps:

[0035] Step 1, drying: dehydrating broccoli at 75°C for 2 hours, and then drying at 55°C to constant weight to obtain dried broccoli;

[0036] Step 2, crushing: crushing the dried broccoli obtained in step 1 to 60 mesh to obtain dry broccoli powder;

[0037] Step 3, preparing enzyme solution: adding myrosinase, cellulase, pectinase, vitamin C and EDTA into a certain amount of water for dissolving and mixing uniformly to obtain an enzyme solution; in this embodiment, myrosinase , The mass ratio between cellulase, pectinase, vitamin C, EDTA and water is 5:2:2:2.8:2.92:10;

[0038] Step 4, enzymatic hydrolysis: add the broccoli dry powder obtained in step 2 to the enzyme solution in step 3, stir evenly, and enzymolyze at 45°C for 3 hours to obtain the enzymatic hydrolysis solution; in this example, broccoli dry powder and enzyme The mass volu...

Embodiment 2

[0045] A kind of method of extracting benzyl isothiocyanate from broccoli of the present embodiment, it may further comprise the steps:

[0046] Step 1, drying: dehydrating the broccoli at 70°C for 3 hours, and then drying at 50°C to constant weight to obtain dried broccoli;

[0047] Step 2, crushing: crushing the dried broccoli obtained in step 1 to 40 mesh to obtain dry broccoli powder;

[0048] Step 3, preparing enzyme solution: adding myrosinase, cellulase, pectinase, vitamin C and EDTA into a certain amount of water for dissolving and mixing uniformly to obtain an enzyme solution; in this embodiment, myrosinase , the mass ratio between cellulase, pectinase, vitamin C, EDTA and water is 4:1:1:1.5:2:9;

[0049] Step 4, enzymatic hydrolysis: add the broccoli dry powder obtained in step 2 to the enzyme solution in step 3, stir evenly, and enzymolyze at 35°C for 5 hours to obtain the enzymatic hydrolysis solution; in this example, broccoli dry powder and enzyme The mass volu...

Embodiment 3

[0056] A kind of method of extracting benzyl isothiocyanate from broccoli of the present embodiment, it may further comprise the steps:

[0057] Step 1, drying: dehydrating broccoli at 80°C for 1 hour, and then drying at 65°C to constant weight to obtain dried broccoli;

[0058] Step 2, crushing: crushing the dried broccoli obtained in step 1 to 80 mesh to obtain dry broccoli powder;

[0059] Step 3, preparing enzyme solution: adding myrosinase, cellulase, pectinase, vitamin C and EDTA into a certain amount of water for dissolving and mixing uniformly to obtain an enzyme solution; in this embodiment, myrosinase , The mass ratio between cellulase, pectinase, vitamin C, EDTA and water is 6:3:3:4:4:12;

[0060] Step 4, enzymatic hydrolysis: add the broccoli dry powder obtained in step 2 to the enzyme solution in step 3, stir evenly, and enzymolyze at 75°C for 1 hour to obtain the enzymatic hydrolysis solution; in this example, broccoli dry powder and enzyme The mass volume rati...

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PUM

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Abstract

The invention relates to the technical field of natural product extraction, in particular to a method for extracting benzyl isothiocyanate from broccoli. The method comprises the steps of 1, drying; 2, grinding; 3, preparing an enzyme solution; 4, carrying out enzymolysis; 5, carrying out ultrasonic extraction; 6, concentrating; 7, extracting and concentrating ethyl ether; 8, carrying out freeze-drying. The method for extracting the benzyl isothiocyanate from the broccoli has the advantages of being high in extraction efficiency and short in extraction time, and 8-12mg of benzyl isothiocyanate crude product can be extracted from per kilogram of broccoli. Furthermore, the method for extracting the benzyl isothiocyanate from the broccoli has the advantages of being environment-friendly, low in energy consumption, good in safety, simple in technology, high in operability and low in production cost.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting benzyl isothiocyanate from broccoli. Background technique [0002] Broccoli (Brassica oleracea L.var.botrytis L), is a 1-2 year-old herbaceous plant of Brassicaceae and Brassica, rich in protein , sugar, fat, vitamins and carotene etc., its nutrient content ranks first among similar vegetables, and it is known as the "crown of vegetables". At the same time, it contains isothiocyanate compounds, so it also has anticancer Efficacy, its efficacy has been recognized by the world and has high medicinal value. [0003] Isothiocyanates are a class of compounds with the general formula R-N=C=S. It widely exists in cruciferous plants, such as: broccoli, cauliflower, kale, etc. The main representatives are: benzyl isothiocyanate, allyl isothiocyanate, sulforaphane, etc., which have effective Anticancer activity. In recent years, studies at ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00
CPCC12P13/00
Inventor 李宝红李福美彭新生王家平
Owner GUANGDONG MEDICAL UNIV