Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins

A lipoxygenase and kit technology, which is applied in the field of 5-lipoxygenase pathway protein combined prediction kit for esophageal cancer patient prognosis, can solve problems such as little research.

Inactive Publication Date: 2016-08-24
CHIFENG UNIV +1
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression of LTA4H in tumor tissues is poorly studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins
  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins
  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] We collected archival wax blocks of surgical specimens from 297 patients with esophageal cancer from 2007 to 2011. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0019] The steps of immunohistochemical hypersensitive two-step method are as follows.

[0020] After the specimens were serially sectioned, dewaxed in xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method, and the specific method was as follows.

[0021] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, they were incubated overnight at 4°C with 5-LO, FLAP, and LTA4H antibodies, respectively.

[0022] (2) The sections were incubated with Polymer enhancer and Polymer polymer respectively and then developed by DAB.

[0023] (3) The sections were counterstained with hemato...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit for detecting expression condition of a paraffin embedding operative specimen of an esophagus cancer patient and further predicting prognosis of the patient by combining 5-lipoxygenase metabolic pathway protein antibodies. The kit comprises three target protein antibodies: 5-LO, FLAP and LTA4H antibodies, and also comprises goat serum, 0.01M citrate repair liquid, 3% H2O2, a Polymer enhancer, a Polymer polymer, a DAB chromogenic reagent and PBS solution. Compared with single index detection, the kit for predicting the prognosis of the patient by combining 5-lipoxygenase metabolic pathway proteins has higher prediction effect, ranking only second to prediction ability of TNM staging. Moreover, the immunohistochemical ultrasensitive two-step method in the invention is a mature and reliable method which can be widely used in primary hospitals.

Description

technical field [0001] The invention relates to a kit for detecting the expression of esophageal cancer patient specimens in combination with 5-lipoxygenase pathway protein antibody and using an immunohistochemical hypersensitive two-step method to predict the prognosis of the patient. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal cancer each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal cancer in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yanting in Sichuan Province, Chaoshan in Guangdong Province, and Huai'an in Jiangsu Province were estab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 白春英张俊毅史铁伟瑞云许秀娥吴健谊许丽艳李恩民
Owner CHIFENG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap