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Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins

A lipoxygenase and kit technology, which is applied in the field of 5-lipoxygenase pathway protein combined prediction kit for esophageal cancer patient prognosis, can solve problems such as little research.

Inactive Publication Date: 2016-08-24
CHIFENG UNIV +1
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Problems solved by technology

The expression of LTA4H in tumor tissues is poorly studied

Method used

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  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins
  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins
  • Kit for predicting prognosis of esophagus cancer patient by combining 5-lipoxygenase pathway proteins

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Experimental program
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Embodiment Construction

[0018] We collected archival wax blocks of surgical specimens from 297 patients with esophageal cancer from 2007 to 2011. Further detailed description will be given below in conjunction with the embodiments of the accompanying drawings.

[0019] The steps of immunohistochemical hypersensitive two-step method are as follows.

[0020] After the specimens were serially sectioned, dewaxed in xylene, and hydrated with graded alcohol, the expression of the target protein was detected by an ultrasensitive two-step method, and the specific method was as follows.

[0021] (1) The slices were repaired with 0.01M citrate repair solution, 3% H 2 o 2 After inactivating endogenous peroxidase and blocking with goat serum, they were incubated overnight at 4°C with 5-LO, FLAP, and LTA4H antibodies, respectively.

[0022] (2) The sections were incubated with Polymer enhancer and Polymer polymer respectively and then developed by DAB.

[0023] (3) The sections were counterstained with hemato...

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Abstract

The invention discloses a kit for detecting expression condition of a paraffin embedding operative specimen of an esophagus cancer patient and further predicting prognosis of the patient by combining 5-lipoxygenase metabolic pathway protein antibodies. The kit comprises three target protein antibodies: 5-LO, FLAP and LTA4H antibodies, and also comprises goat serum, 0.01M citrate repair liquid, 3% H2O2, a Polymer enhancer, a Polymer polymer, a DAB chromogenic reagent and PBS solution. Compared with single index detection, the kit for predicting the prognosis of the patient by combining 5-lipoxygenase metabolic pathway proteins has higher prediction effect, ranking only second to prediction ability of TNM staging. Moreover, the immunohistochemical ultrasensitive two-step method in the invention is a mature and reliable method which can be widely used in primary hospitals.

Description

technical field [0001] The invention relates to a kit for detecting the expression of esophageal cancer patient specimens in combination with 5-lipoxygenase pathway protein antibody and using an immunohistochemical hypersensitive two-step method to predict the prognosis of the patient. Background technique [0002] Esophageal squamous cell carcinoma (ESCC) is a common malignant tumor of the upper gastrointestinal tract in my country, ranking fourth in the mortality rate of malignant tumors in China. Of the more than 400,000 new cases of esophageal cancer each year in the world, more than half occur in my country, which is also recognized as the country with the highest incidence of esophageal cancer in the world. In the 1970s, a nationwide malignant tumor survey was carried out, and Cixian County in Hebei Province, Lin County in Henan Province, Yangquan in Shanxi Province, Yanting in Sichuan Province, Chaoshan in Guangdong Province, and Huai'an in Jiangsu Province were estab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 白春英张俊毅史铁伟瑞云许秀娥吴健谊许丽艳李恩民
Owner CHIFENG UNIV
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