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Implication type solid-phase logic gate based on BSA (bull serum albumin)/3-MPA (mercaptopropionic acid)-gold nanocluster-polyelectrolyte and construction method of implication type solid-phase logic gate

A 3-MPA-, gold nanocluster technology, applied in the nano field, can solve the problems of high toxicity, poor photostability, poor portability, etc.

Active Publication Date: 2016-08-31
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the logic operation systems have the following disadvantages: (1) involve complex modification or labeling process, and the cost is high; (2) they are not resettable; (3) they are poor in portability and difficult to attach to solid surfaces; (4) ) cannot handle multiple input signals at the same time and the integration between logic gates is very difficult
Although many fluorescent dyes have been reported to perform logic operations, the problems of high cost, poor photostability, prone to autoxidation, and high toxicity limit their further applications.

Method used

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  • Implication type solid-phase logic gate based on BSA (bull serum albumin)/3-MPA (mercaptopropionic acid)-gold nanocluster-polyelectrolyte and construction method of implication type solid-phase logic gate
  • Implication type solid-phase logic gate based on BSA (bull serum albumin)/3-MPA (mercaptopropionic acid)-gold nanocluster-polyelectrolyte and construction method of implication type solid-phase logic gate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of BSA / 3-MPA-gold nanocluster fluorescent material: Mix 2.5 mL bovine serum albumin with a concentration of 50 mg / mL and 2.5 mL chloroauric acid solution with a concentration of 10 mmol / L, and then add 0.25 mL concentration 1 mol / L sodium hydroxide solution and 0.25 mL 4 mol / L 3-mercaptopropionic acid solution were shaken and mixed, and reacted at 4 °C for 1 hour, and the reaction solution changed from light yellow to colorless. The reaction solution was dialyzed in 20 mmol / L pH 3 phosphate buffer for 48 hours with a dialysis bag with a molecular weight cut-off of 7000, and then dialyzed in deionized water for 12 hours to obtain a BSA / 3-MPA-gold nanocluster solution. Stored in the dark at 4 °C, it can remain relatively stable for at least two months.

Embodiment 2

[0033] Preparation of BSA / 3-MPA-gold nanoclusters-polyelectrolyte solid-phase composite: 0.5 mL of 0.5 mol / L sodium chloride solution (containing 10 mg / mL polydiallyldimethylammonium chloride ) was added to 0.5 mL of the gold nanocluster solution prepared in Example 1, and ultrasonicated for 15 seconds; then 1 mL of 0.5 mol / L sodium chloride solution (containing 10 mg / mL sodium polystyrene sulfonate) was added , sonicate for 1 min, and vortex for 30 min to obtain a white colloidal dispersion. 25 μL of the prepared colloidal dispersion was drop-coated on a glass slide, then placed in an oven at 37 °C, and taken out after 30 minutes to obtain a gold nanocluster-polyelectrolyte solid-phase composite.

Embodiment 3

[0035] Add 10 mL of 50 mmol / L acetate buffer solution with pH=4.6 to the BSA / 3-MPA-gold nanoclusters-polyelectrolyte solid-phase composite prepared in Example 2, react at room temperature for 10 minutes, and uv lamp 302 nm Observed under the wavelength, the BSA / 3-MPA-gold nanocluster-polyelectrolyte solid phase complex has orange-yellow fluorescence. That is, when the input signal is (0, 0), the output signal is 1 (see figure 1 ).

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Abstract

The invention discloses an implication type solid-phase logic gate based on BSA (bull serum albumin) / 3-MPA (mercaptopropionic acid)-gold nanocluster-polyelectrolyte and a construction method of the implication type solid-phase logic gate. On the basis of the phenomenon that H+ can inhibit fluorescence quenching of Fe2+ to a BSA / 3-MPA-gold nanocluster-polyelectrolyte solid-phase compound in an acetic acid buffer solution with the pH value of 4.6, the Fe2+ and the H+ are taken as input signals, the gold nanocluster-polyelectrolyte solid-phase compound serves as a signal converter, and an implication type solid-phase logic gate system is created. The logic gate system has the advantages of simplicity and convenience in operation (without any modifying and marking processes), high transferability, capability of being reset, diversification in signal reading and the like, and has good application prospect in the fields of clinical diagnosis, chemical sensing, environment monitoring and the like.

Description

technical field [0001] The invention relates to an IMPLICATION solid-phase logic gate based on BSA / 3-MPA-gold nano cluster-polyelectrolyte complex and a construction method thereof, belonging to the field of nanotechnology. Background technique [0002] In the past few decades, chemical Boolean logic gates have attracted extensive attention and have been applied in many fields such as clinical diagnosis, chemical sensing, and environmental monitoring. At present, different types of logic gates, such as AND, OR, NOR, NAND, IMPLICATION, and INHIBIT, can be constructed by using different materials (such as nucleic acids, enzymes, organic molecules, and nanomaterials). However, most of the logic operation systems have the following disadvantages: (1) involve complex modification or labeling process, and the cost is high; (2) they are not resettable; (3) they are poor in portability and difficult to attach to solid surfaces; (4) ) cannot process multiple input signals at the sam...

Claims

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Application Information

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IPC IPC(8): G01N21/64G02F3/00G06F19/00
CPCG01N21/6428G01N2021/6432G02F3/00G16C20/10
Inventor 陈伟王菲菲邓豪华郑晓晴李东铃
Owner FUJIAN MEDICAL UNIV
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