Traditional Chinese medicine common pecteilis root extract capable of improving production performance of cattle
A technology for producing performance and rabbit ear grass, which is applied in the field of veterinary medicine and animal husbandry to achieve the effect of simple preparation method and improving the production performance of cattle
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Embodiment 1
[0015] Example 1: Preparation of Rabbit Ear Grass Extract
[0016] (a) Mix 5 kg of dried rabies ear grass and bindweed (accounting for 10% of the total weight), grind them, and extract them under reflux with 70% ethanol solution (20 L x 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol Extract (3L×3 times), concentrate under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract is dissolved in water, filtered, and filtered with AB-8 To enrich the active ingredient in the resin, first wash 8 column volumes with 8% ethanol to remove large polar components, then elute 15 column volumes with 75% ethanol, collect the 75% ethanol e...
Embodiment 2
[0017] Example 2: Preparation of Rabbit Ear Grass Extract
[0018] (a) Mix 5kg of dried Rabbit's Ear Grass and Convolvulus convolvulus (accounting for 5% of the total weight), pulverize, extract with 65% ethanol solution under heat reflux (20L x 3 times), combine the extracts, concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol Extract (3L×3 times), concentrate under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (c) n-butanol extract is dissolved in water, filtered, and filtered with AB-8 To enrich the active ingredient in the resin, wash 9 column volumes with 6% ethanol to remove large polar components, then elute 17 column volumes with 70% ethanol, collect the 70% ethano...
Embodiment 3
[0019] Example 3: Preparation of Rabbit Ear Grass Extract
[0020] (a) Mix 5kg of dried rabies ear grass and bindweed (accounting for 15% of the total weight), pulverize, extract with 75% ethanol solution under heat reflux, combine the extracts (20L×3 times), concentrate until there is no alcohol smell to obtain ethanol Extract the concentrated solution; (b) dilute the ethanol extracted concentrated solution obtained in step (a) with water (3L), and successively use petroleum ether (3L×3 times), ethyl acetate (3L×3 times) and water-saturated n-butanol (3L×3 times) extraction, concentration under reduced pressure to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; To enrich the active ingredients in the resin, wash 7 column volumes with 10% ethanol to remove large polar components, then elute 13 column volumes with 80% ethanol, collect the 80% ethanol eluate, and concentrate under reduced pressure.
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