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Bionic tissue based on acellular matrix-cell-three-dimensional fiber network and preparation method of bionic tissue

An acellular matrix and fiber network technology, applied in the field of composite acellular matrix hydrogel preparation, can solve the problems of insufficient structural strength, fast migration speed of effective added components, etc., and achieve good biocompatibility and good biodegradability. Effect

Active Publication Date: 2016-09-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a biomimetic tissue based on acellular matrix-cell-three-dimensional fiber network and its preparation method, so as to solve the problems of insufficient structural strength and excessive migration speed of internal effective added components in the prior art question

Method used

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  • Bionic tissue based on acellular matrix-cell-three-dimensional fiber network and preparation method of bionic tissue
  • Bionic tissue based on acellular matrix-cell-three-dimensional fiber network and preparation method of bionic tissue

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Embodiment 1

[0091] A method for preparing a bionic tissue of acellular matrix-cell-three-dimensional fiber network, first of all, preparing a composite acellular matrix hydrogel, preparing the acellular matrix material from the urethra, freeze-drying and then freeze-grinding. Crumble into powder; digest the decellularized matrix powder with pepsin in hydrochloric acid solution for about 48 hours to form the decellularized matrix pregel solution, add NaOH solution about 1 / 10 of the volume of the decellularized matrix pregel solution, wherein the concentration of NaOH solution 0.01mol / L, then add about 1 / 9 of the volume of acellular matrix pregel solution 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add insulin, wherein the mass ratio of insulin to acellular matrix is ​​0.05 :100, forming the acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned acellular matrix precursor solution was coated on the fiber prepared by the three-dimensional weav...

Embodiment 2

[0093] A method for preparing a bionic tissue of acellular matrix-cell-three-dimensional fiber network. Firstly, the composite acellular matrix hydrogel is prepared, and the acellular matrix material obtained from the ureter is prepared, freeze-dried, then freeze-ground, and crushed. Crumble into powder; digest the decellularized matrix powder with pepsin in hydrochloric acid solution for about 48 hours to form the decellularized matrix pregel solution, add NaOH solution about 1 / 10 of the volume of the decellularized matrix pregel solution, wherein the concentration of NaOH solution 0.01mol / L, then add about 1 / 9 of the volume of acellular matrix pregel solution 10×PBS, wherein the concentration of PBS is 0.01mol / L, then add aspirin, wherein the mass ratio of aspirin to acellular matrix is ​​1 :20, forming the acellular matrix gel precursor solution. Before the gel was formed, the above-mentioned acellular matrix precursor solution was sprayed on the fiber prepared by the three...

Embodiment 3

[0095] A method for preparing a bionic tissue of acellular matrix-cell-three-dimensional fiber network, first of all, preparing a composite acellular matrix hydrogel, preparing the acellular matrix material from the bladder, freeze-drying and then freeze-grinding. Crumble into powder; digest the decellularized matrix powder with pepsin in hydrochloric acid solution for about 48 hours to form the decellularized matrix pregel solution, add NaOH solution about 1 / 10 of the volume of the decellularized matrix pregel solution, wherein the concentration of NaOH solution 0.01mol / L, then add 10×PBS of about 1 / 9 volume of the decellularized matrix pregel solution, wherein the concentration of PBS is 0.01mol / L, then add ketoprofen, wherein ketoprofen and decellularized matrix The mass ratio is 1:10 to form the acellular matrix gel precursor solution. Before the gel is formed, the above-mentioned acellular matrix precursor solution is coated on the fibers prepared by the three-dimensional ...

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Abstract

The invention relates to bionic tissue based on an acellular matrix-cell-three-dimensional fiber network and a preparation method of the bionic tissue. The bionic tissue based on the acellular matrix-cell-three-dimensional fiber network at least comprises composite acellular matrix hydrogel and epithelial cells. The composite acellular matrix hydrogel adopts a three-dimensional fiber network material good in biocompatibility as a framework. The three-dimensional fiber network material good in biocompatibility is a network structure material formed by winding fibers good in biocompatibility. The bionic tissue based on the acellular matrix-cell-three-dimensional fiber network can carry out simulation according to the tissue and organ structures, has good application prospects in tissue engineering, and can be widely applied to the field of biomedicine.

Description

technical field [0001] The invention belongs to the technical field of composite acellular matrix hydrogel preparation, and relates to a bionic tissue based on an acellular matrix-cell-three-dimensional fiber network and a preparation method thereof. Background technique [0002] Hydrogel (Hydrogel) is a gel with water as the dispersion medium, a polymer network system, soft in nature, similar to extracellular matrix in nature, because it can change shape at will, its strength can be adjusted, and its interior is loose and porous. Properties are increasingly being used in biological tissue engineering. [0003] All water-soluble or hydrophilic polymers can form hydrogels through certain chemical cross-linking or physical cross-linking. These polymers can be divided into two categories according to their sources, natural and synthetic. Natural hydrophilic polymers include polysaccharides (starch, cellulose, alginic acid, hyaluronic acid, chitosan, etc.) and polypeptides (co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/22A61L27/18A61L27/50
CPCA61L27/18A61L27/225A61L27/3633A61L27/3813A61L27/50C08L89/00C08L67/04
Inventor 张耀鹏蒋丹宋鲁杰邵惠丽黄建文傅强胡学超
Owner DONGHUA UNIV
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