Cell screening chip and microfluidic combined chip

A microfluidic chip and cell technology, applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of low capture rate, low screening characteristics, low screening throughput, etc. Achieve the effects of increasing purity, high-throughput screening, and saving manpower and material resources

Active Publication Date: 2016-09-21
牛海涛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a cell screening chip integrating physical screening and antibody affinity screening, which overcomes the technical defects of low screening throughput, low capture rate and low screening charact

Method used

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  • Cell screening chip and microfluidic combined chip
  • Cell screening chip and microfluidic combined chip
  • Cell screening chip and microfluidic combined chip

Examples

Experimental program
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Effect test

Embodiment 1

[0055] like Figure 14 Shown is a structure of the cell culture unit D, including three juxtaposed cell culture chambers D310, D320, and D330, each cell culture chamber is provided with a cell inlet and a cell outlet, the cell culture chamber is quadrilateral, and there are 4- 8 support columns. The biological glue perfusion unit D200 surrounds the periphery of each cell culture chamber D310, D320, and D330, pours the matrigel through the bioglue inlet D201, fills the entire colloid channel and microbridge, and surrounds the cell culture chamber through the glue outlet D202 discharge. The nutrient solution perfusion unit D100 is surrounded by the periphery of the biological glue perfusion unit D200, the nutrient solution is poured into the nutrient solution inlet D101, and a negative pressure suction device (which can be a negative pressure suction micro-syringe) is provided at the nutrient solution outlet D102 for nutrition. The liquid perfusion provides negative pressure s...

Embodiment 2

[0059] The bladder cancer tumor cells are screened and cell cultured by using the microfluidic combined chip.

[0060] Before the experiment, close the valve C, coat the avidin-biotin into the second screening chamber B3 through the antibody infusion port B1, and then infuse the bladder cancer monoclonal antibody BCMab1 into the affinity chamber through the antibody infusion port B1 Sex screening unit, coated with antibodies. At the same time, at 0-4°C, the matrigel was poured into the bioglue perfusion unit to fill the colloid flow channel and each microbridge, and maintained at 37°C for 8-12h, and it began to be gelatinous. At the same time, the tumor sample cells are pre-labeled and processed to make a mixture of dilution gel and cells. The concentration of cells in the dilution gel is 10 6 -10 7 / ml.

[0061] BCMab1 is a highly specific anti-bladder cancer monoclonal antibody that can recognize abnormally glycosylated integrin a3b1 and is a bladder cancer tumor marker....

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Abstract

The invention discloses a cell screening chip and a microfluidic combined chip. The cell screening chip comprises a physical screening unit and an affinity screening unit; the physical screening unit comprises n first screening chambers connected in parallel, n is larger than or equal to one, multiple rows of stand columns are arranged in a screening pipeline, the staggered distance d between every two adjacent stand columns is equal to 1/3 lambda, the gap G width between every two adjacent stand columns in the same row is equal to lambda/2R, and the included angle alpha between the stand columns in the previous row and the stand columns in the next row is equal to arctan1/3, wherein lambda represents the central distance between two adjacent stand columns, and R represents the diameter of the stand columns; the affinity screening unit comprises a second screening chamber, an inlet of a first discharging channel is connected with the physical screening unit, and an outlet of the first discharging channel is connected with the affinity screening unit. The microfluidic combined chip comprises the physical screening unit, the affinity screening unit, a cell culture unit and a valve. Phenotype of various cells can be observed in real time, the step of using large equipment for separating heterogeneous cells is omitted, and manpower and material resources are saved.

Description

technical field [0001] The invention belongs to the technical field of cell screening and cell culture, and in particular relates to a microfluidic chip for cell screening and a microfluidic combined chip capable of simultaneously realizing cell screening and cell culture. Background technique [0002] At present, microfluidic chips are widely used in the medical field by virtue of their advantages of low cost and small equipment. In particular, microfluidic technology has the advantages of less sample consumption, high resolution and sensitivity, easy integration and miniaturization, and has been widely used in the study of circulating tumor cell sorting, and has shown good development prospects. [0003] The current tumor cell screening methods include passive sorting methods, active sorting methods and multiple sorting methods. The passive sorting method is based on the different physical and biological characteristics of different types of cells such as size, density, s...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M23/16C12M23/34C12M47/04
Inventor 牛海涛王云超王建宁
Owner 牛海涛
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