Fungus fluorescent staining agent

A technology of fluorescent dyeing and fluorescent whitening agent, which is applied in the field of fungal fluorescent dyes, can solve the problems of reduced detection sensitivity, long dyeing time, and inability to fungus, and achieve the goals of improving fungal detection efficiency, simplifying specimen staining steps, and good staining effects Effect

Active Publication Date: 2016-09-21
南京汉瑞生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is due to the poor stability of the fluorescent whitening agent and the background dye after they are miscible and placed, which will cause a decrease in detection sensitivity and even make it impossible to stain fungi. Therefore, the staining solution needs to be prepared and stored separately, and two steps are required for stai

Method used

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  • Fungus fluorescent staining agent
  • Fungus fluorescent staining agent
  • Fungus fluorescent staining agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 fungal fluorescent dye

[0032] Reagent 1:

[0033] Solution A: Dissolve the fluorescent whitening agent 28 in a solution with a concentration of 10% (W / V) potassium hydroxide, and dissolve sufficiently so that the concentration of the fluorescent whitening agent is 0.1% (W / V).

[0034] Solution B: 1% Evans blue solution.

[0035] Before use, it is necessary to stain the sample with solution A, and then use solution B to stain the sample.

[0036] Reagent 2:

[0037] Fluorescent whitening agent 28 is dissolved in the solution that concentration is 10% (W / V) potassium hydroxide, and dissolving is sufficient, and the concentration that makes fluorescent whitening agent 28 is 0.1% (W / V), adds Evans blue dyestuff and Glycerol was used so that the concentration of Evans blue dye was 1% (W / V), and the concentration of glycerol was 30% (V / V).

[0038] Reagent 3:

[0039] Fluorescent whitening agent 28 is dissolved in the solution that conce...

Embodiment 2

[0044] The staining of embodiment 2 dander specimens

[0045] Select 5 dander specimens with similar size, shape and weight, place them on glass slides respectively, add a drop of ethanol to fix the tissue sections, leave them at room temperature for 5 minutes, wash the sections with water, blot the excess water with paper, and use reagent 1, 2,3,4,5 Stain for 1 minute, add a cover slip, absorb excess dye with paper, and then observe under a fluorescent microscope. Activated with 340nm–400nm UV light, the fungus will appear blue or yellowish green. The results showed that all the reagents could stain the fungus in the dander specimens. The dyeing effect is not much different, and the time required for dyeing is basically the same. Taking reagent 1 and reagent 3 as an example, the staining results of the two are as follows figure 1 As shown, the results show that both of them can stain the fungus in dander specimens, but the background contrast of reagent 3 to fungal stainin...

Embodiment 3

[0046] The dyeing of embodiment 3 foot skin samples

[0047] Select 5 small pieces of foot skin specimens with similar size, shape and weight, place them on glass slides, stain with reagents 1, 2, 3, 4, and 5 respectively, and leave them at room temperature for staining. If the foot skin cannot be stained, the staining can be extended appropriately time, or increase the dyeing temperature to speed up the dyeing of the foot skin. A cover slip was added and observed under a fluorescent microscope. Activated with 340nm–400nm UV light, the fungus will appear blue or yellow-green. The results are shown in Table 1. The results show that reagents 3, 4, and 5 have the fastest staining speed, and fungi can be observed after 3 minutes of staining, while reagent 1 has the slowest staining speed, and only a small amount of fungi can be seen after 30 minutes of staining. It shows that the sensitivity of reagent 1 is not as high as that of reagents 2, 3, 4, and 5. Taking reagent 1 and re...

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Abstract

The invention discloses a fungus fluorescent staining agent, which consists of a fluorescent whitening agent, a background dye and a stabilizing component. The finished product (the fungus fluorescent staining agent) disclosed by the invention is good in stability and is capable of achieving one-drop rapid staining; and the product, which is not limited by specimens, is applicable to large and deep specimens, so that the efficiency of fungus detection is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biomedical diagnosis, and in particular relates to a fungal fluorescent dye. Background technique [0002] With the development of today's society, the pace of life is accelerating, and the number of people with low immunity is increasing year by year, so that the infection caused by fungi is becoming more and more prominent. According to WHO statistics, there are more than 270 species of fungi that can cause human diseases, which can be divided into superficial fungi and deep fungi according to the infection site. Superficial fungi attack the skin, hair, and nails, while deep fungi infect various organs and systems of the human body such as the heart, lungs, blood, and gastrointestinal tract, endangering the lives of patients. At present, the effective means of treating fungal infection is still antibiotic drug treatment. Accurately diagnosing the cause of the infection is critical to selecting the app...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/04C12Q2304/10
Inventor 柳丽赖增祖叶欣
Owner 南京汉瑞生物科技有限公司
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