Unlock instant, AI-driven research and patent intelligence for your innovation.

Water-jacketed incubator high temperature sterilization method

A high-temperature sterilization and incubator technology, applied in heating and other directions, can solve the problems of secondary pollution, inability to remove microorganisms, and difficult to guarantee the disinfection effect, and achieve the effect of improving the success rate

Active Publication Date: 2019-01-18
上海力申科学仪器有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effectiveness of the ultraviolet lamp irradiation method is affected by many factors, such as occlusion, time, intensity, irradiation distance, humidity, etc., and the disinfection effect is difficult to guarantee; Wiping and other factors lead to limited disinfection effect; while HEPA filtration cannot remove the growing microorganisms and may cause secondary pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Water-jacketed incubator high temperature sterilization method
  • Water-jacketed incubator high temperature sterilization method
  • Water-jacketed incubator high temperature sterilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1, ambient temperature: 30°C; heating time: 10:30~12:45

[0061]

[0062] Table 1

[0063] illustrate:

[0064] 1) It takes 2 hours and 15 minutes for the temperature in the center of the inner tank of the incubator to rise from 30°C to 90°C;

[0065] 2) When the temperature of the inner liner center of the incubator cavity rises to 90°C, after the heating unit 10 is powered off, the inner liner temperature of the incubator cavity rises to a maximum of 90.9°C.

Embodiment 2

[0066] Example 2, ambient temperature: 29°C; heating time: 10:15~13:15

[0067]

[0068] Table 2

[0069] illustrate:

[0070] 1) It takes 3 hours for the temperature in the center of the inner tank of the incubator to rise from 29°C to 90°C;

[0071] 2) When the temperature of the inner liner center of the incubator cavity rises to 90° C., after the heating unit 10 is powered off, the inner liner temperature of the incubator cavity rises to a maximum of 90.4° C.

Embodiment 3

[0072] Example 3, ambient temperature: 34°C; heating time: 09:30~11:00

[0073]

[0074] table 3

[0075] illustrate:

[0076] 1) It takes 1 hour and 30 minutes for the temperature of the inner tank center to rise from 36°C to 90°C (the water in the water jacket has been released one day ago);

[0077] 2) When the inner tank center temperature rises to 90°C, after the heating cable is powered off, the inner tank center temperature rises up to 90.5°C;

[0078] 3) It takes 2 hours for the inner tank center temperature to drop from 90°C to 60°C after power off;

[0079] 4) Circulating water with a central temperature of 60°C in the inner tank (a total of 40 liters of circulating water) enters the water jacket to start circulating cooling, and it takes 42 minutes for the central temperature of the inner tank to drop to 37°C.

[0080] Measure the temperature of the bottom, back, left side, right side, top, glass door and the center point, upper wall, lower wall, left wall, righ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a high-temperature sterilization method of a water-jacket type incubator. The high-temperature sterilization method comprises the following steps: 1, promoting water in a water jacket to flow into a water tank by virtue of a water jacket water outlet and a water tank water inlet; 2, heating the water jacket which no water exists in the water jacket; 3, stopping heating the water jacket when temperature of an inner cavity of an incubator body of the incubator rises to a preset value, and keeping the temperature of the inner cavity of the incubator body at the preset value temperature for high-temperature sterilization; 4, after the high-temperature sterilization, when the inner cavity of the incubator is naturally cooled to a first preset temperature, turning on the water pump, the water jacket water outlet and the water tank water inlet, so that the water flows between the water jacket and the water tank in a circulating mode; and 5, after the temperature of the inner cavity of the incubator body drops to a second preset temperature, fully filling a liner with pure water. With the application of the high-temperature sterilization method of the water-jacket type incubator disclosed by the invention, high-temperature dry heat air sterilization or wet heat sterilization is achieved; and the defect that an existing water-jacket type carbon dioxide incubator fails to achieve high-temperature sterilization is avoided, and the success rate of cultivation research experiments is greatly improved.

Description

technical field [0001] The invention mainly relates to a sterilization method, in particular to a high-temperature sterilization method for a water-jacketed incubator. Background technique [0002] The carbon dioxide incubator is to form a growth environment similar to cells / tissues in organisms by simulating in the incubator box, such as stable temperature (37°C), stable CO 2 Level (5%), constant pH (PH value: 7.2-7.4), high relative saturation humidity (≥95%), a device for in vitro culture of cells / tissues. Widely used in the culture of cells, tissue culture and some special microorganisms, commonly used in cell dynamics research, collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, and culture Antibody production by hybridoma cells, in vitro fertilization (IVF), stem cells, tissue engineering, drug screening and other research fields. [0003] Carbon dioxide incubato...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/04A61L2/06
Inventor 吴峻张云春孙大炜
Owner 上海力申科学仪器有限公司