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High temperature sterilization water jacket incubator

A high-temperature sterilization, water-jacketed technology, applied in the direction of sterilization methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of secondary pollution, difficult to guarantee disinfection effect, and inability to remove microorganisms, etc., to achieve The effect of increasing the success rate

Active Publication Date: 2018-05-11
上海力申科学仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effectiveness of the ultraviolet lamp irradiation method is affected by many factors, such as occlusion, time, intensity, irradiation distance, humidity, etc., and the disinfection effect is difficult to guarantee; Wiping and other factors lead to limited disinfection effect; while HEPA filtration cannot remove the growing microorganisms and may cause secondary pollution

Method used

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  • High temperature sterilization water jacket incubator
  • High temperature sterilization water jacket incubator
  • High temperature sterilization water jacket incubator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Ambient temperature: 30°C; heating time: 10:30-12:45

[0066]

[0067]

[0068] Table 1

[0069] Description:

[0070] 1) It takes 2 hours and 15 minutes for the temperature of the bile center in the chamber of the incubator to rise from 30°C to 90°C;

[0071] 2) When the temperature of the bile center in the chamber of the incubator rises to 90°C, after the heating unit 10 is powered off, the temperature of the bile center in the chamber of the incubator rises to a maximum of 90.9°C.

Embodiment 2

[0072] Example two, ambient temperature: 29°C; heating time: 10:15 to 13:15

[0073]

[0074] Table 2

[0075] Description:

[0076] 1) It takes 3 hours for the temperature of the bile center in the chamber of the incubator to rise from 29°C to 90°C;

[0077] 2) When the temperature of the bile center in the chamber of the incubator rises to 90°C, after the heating unit 10 is powered off, the temperature of the bile center in the chamber of the incubator rises to a maximum of 90.4°C.

Embodiment 3

[0078] Example three, ambient temperature: 34°C; heating time: 09:30-11:00

[0079]

[0080] table 3

[0081] Description:

[0082] 1) It takes 1 hour and 30 minutes for the inner temperature of the inner tank to rise from 36℃ to 90℃ (the water in the water jacket has been released one day ago);

[0083] 2) When the center temperature of the inner tank rises to 90°C, after the heating cable is powered off, the center temperature of the inner tank rises to 90.5°C;

[0084] 3) It takes 2 hours for the inner temperature of the inner tank to drop from 90℃ to 60℃ after power failure;

[0085] 4) Circulating water (40 liters of circulating water) at the center of the inner tank at 60°C enters the water jacket to begin circulating cooling, and it takes 42 minutes for the center temperature of the inner tank to drop to 37°C.

[0086] Measure the temperature of the bottom, back, left side, right side, top, glass door and inner cavity center, upper wall, lower wall, left wall, right wall and glass ...

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PUM

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Abstract

The high-temperature sterilization water-jacketed incubator of the present invention comprises: a box body; a water jacket, the water jacket is arranged in the cavity of the box body, and a water jacket water outlet and a water jacket water inlet are arranged on the water jacket; a water tank, the water tank is arranged in In the cavity of the tank body, the water tank is provided with a water tank water inlet and a water tank water outlet; the circulation unit is connected with the water jacket and the water tank through the water jacket water inlet and the water tank water outlet respectively, and an external water inlet is provided on the circulation unit ; heating unit, the heating unit is arranged outside the water jacket; insulation layer, the insulation layer is arranged outside the water jacket and the heating unit; the control device, the control device is arranged in the box. The invention can realize high-temperature dry-hot air sterilization or damp-heat sterilization, can make up for the shortcomings of current water-jacketed incubators that cannot realize high-temperature sterilization, greatly reduces microbial pollution in the use environment inside the incubator, and greatly improves the efficiency of cultivation research experiments. Success rate.

Description

Technical field [0001] The present invention mainly relates to a carbon dioxide incubator. The present invention belongs to experimental instruments and equipment, and particularly relates to a water-jacketed carbon dioxide incubator that can realize high temperature sterilization. Background technique [0002] The carbon dioxide incubator simulates the growth environment of cells / tissues in the organism by simulating in the incubator, such as stable temperature (37°C) and stable CO 2 Level (5%), constant pH (PH value: 7.2~7.4), high relative saturation humidity (≥95%), a device for cell / tissue culture in vitro. Widely used in cell, tissue culture and the cultivation of some special microorganisms, commonly used in cell dynamics research, mammalian cell secretion collection, carcinogenic or toxicological effects of various physical and chemical factors, antigen research and production, and culture Hybridoma cells produce antibodies, in vitro fertilization (IVF), stem cells, tissu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36C12M1/34C12M1/12C12M1/00
Inventor 吴峻张云春孙大炜
Owner 上海力申科学仪器有限公司