Active substance for inducing autolysis of microalgae cells and method for producing the same

Abstract

The present invention provides an active substance that induces microalgae cell autolysis and a manufacturing method thereof, comprising: inoculating a bacterial strain of the genus Bacillus in a culture solution to obtain a bacterium suspension; The bacterial strains of the genus Bacillus are cultivated at least until the growth stationary phase, until the bacterial strains of the genus Bacillus form aggregates in the bacterium suspension and the bacterium suspension becomes clear; and the bacterial strains of the genus Bacillus in the After aggregation is formed in the cell suspension and the cell suspension becomes clear, the cell suspension is subjected to a vacuum distillation procedure to obtain an active solution, wherein the active solution contains an activity that induces the self-lysis of microalgae cells substance.

Description

technical field [0001] The invention relates to an active substance inducing microalgae cell autolysis and a manufacturing method thereof, as well as the application of the active substance inducing microalgae cell autolysis. Background technique [0002] In order to solve the current problems of global warming caused by the excessive use of fossil energy and the increase in the cost of petroleum utilization, the conversion of biomass sources into energy has been regarded as one of the ways to replace traditional fossil fuels. In the development of biomass energy technology, microalgae biodiesel is a research direction that has attracted attention. [0003] Different from the current industry that mainly applies microalgae to high-priced products such as food, health care, and biomedicine, the application of microalgae to the biofuel industry has a large market size, but the unit price of the product is low, and more emphasis is placed on production costs and energy consumpt...

AI Technical Summary

Problems solved by technology

In order to remove oil from microalgae cells as biofuel, microalgae cells must be destroyed first, but this process is often quite energy-intensive, resulting in increased costs

[0004] In past studies, it was found that destroying the cell wall of microalgae with traditional mechanical force needs to consume about 30% of the total energy contained in the microalgae cell, which has become a major obstacle to the industrialization of microalgae biofuels

The chemical wall-breaking method requires the use of chemical agents, so while destroying biological cells, it may also cause damage to intracellular products, which is not conducive to the subsequent product recovery and use. Moreover, chemical wall-breaking also requires additional stirring power, which increases the cost. improve

[0005] In view of the fact that the traditional mechanical wall breaking technology consumes too much energy to treat high-water biomass such as microalgae cells, it does not meet the needs of the biofuel industry, and chemical wall breaking can easily cause damage to intracellular products and still require additional stirring power , so there is an urgent need for a novel low-energy, low-cost microalgae wall-breaking technology, in the hope that microalgae biomass energy can be marketed

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